CCL7 ELISA Kit

Produktnummer ABIN6720040

Hersteller Produkt- Nr.: koa0252

Produktdetails CCL7 ELISA Kit

Target: CCL7 (Chemokine (C-C Motif) Ligand 7 (CCL7))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.6 pg/mL - 1000 pg/mL

Untere Nachweisgrenze: 15.6 pg/mL

Applikation: ELISA

Hersteller Produkt- Nr.: koa0252

Hersteller: Rockland

Verwendungszweck: Human CCL7 - MCP-3 Sandwich ELISA Kit for Quantitative Detection

Marke: AccuSignal™

Proben: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytische Methode: Quantitative

Spezifität:

Production: Natural and recombinant human CCL7. There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 1 pg/mL

Bestandteile:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Anwendungsinformationen

Applikationshinweise: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human CCL7 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human CCL7 standard solution and each sample be measured in duplicate.

Kommentare:

Standard: Expression system for standard: E.coli, Immunogen sequence: Q24-L99

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu CCL7

Target: CCL7 (Chemokine (C-C Motif) Ligand 7 (CCL7))

Andere Bezeichnung: CCL7/MCP-3 (CCL7 Produkte)

Hintergrund:

Synonyms: C-C motif chemokine 7, Ccl7, CCL7_HUMAN, Chemokine CC Motif Ligand 7, Chemotactic protein 3, FIC, Intercrine/chemokine MARC, MARC, MCP 3, MCP-3, Monocyte chemoattractant protein 3, Monocyte chemotactic protein 3, NC28, RANTES/sis homolog, RP23-350G1.4, SCYA6, SCYA7, Small inducible cytokine A7, Small-inducible cytokine A7

Background: Chemokine(C-C motif) ligand 7(CCL7) is a small cytokine known as a chemokine that was previously called monocyte-specific chemokine 3(MCP3). It belongs to the C-C chemokine family. By fluorescence in situ hybridization, mapped the MCP3 gene to chromosome 17q11.2-q12. MCP3 was identified as a physiologic substrate of gelatinase A. Cleaved MCP3 binds to CC-chemokine receptors-1, -2, and -3, but no longer induces calcium fluxes or promotes chemotaxis, and instead acts as a general chemokine antagonist that dampens inflammation, suggested that matrix metalloproteinases are both effectors and regulators of the inflammatory response.

Gen-ID: 6354

NCBI Accession: NP_006264

UniProt: P80098