CCL8 ELISA Kit

Produktnummer ABIN6720041

Produktdetails CCL8 ELISA Kit

Target: CCL8 (Chemokine (C-C Motif) Ligand 8 (CCL8))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.6 pg/mL - 1000 pg/mL

Untere Nachweisgrenze: 15.6 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich ELISA for Quantitative Detection of Antigen

Proben: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytische Methode: Quantitative

Produktmerkmale:

Synonyms: C C motif chemokine 8, CCL 8, Ccl8, CCL8_HUMAN, Chemokine (C C motif) ligand 8, Chemokine ligand 8, HC14, MCP 2, MCP-2, MCP-2(6-76), Monocyte chemoattractant protein 2, Monocyte chemotactic protein 2, SCYA10, SCYA8, Small inducible cytokine A8, Small inducible cytokine subfamily A (Cys Cys) member 8, Small-inducible cytokine A8

Background: Chemokine(C-C motif) ligand 8, also known as monocyte chemoattractant protein 2(MCP-2), is a protein that in humans is encoded by the CCL8 gene. CCL8 is a small cytokine belonging to the CC chemokine family. The CCL8 protein is produced as a precursor containing 109 amino acids, which is cleaved to produce mature CCL8 containing 75 amino acids. The gene for CCL8 is encoded by 3 exons and is located within a large cluster of CC chemokines on chromosome 17q11.2 in humans. Monocyte chemotactic protein 2(MCP-2) is a CC chemokine that utilizes multiple cellular receptors to attract and activate human leukocytes.

Gene Name: CCL8

Production: Natural and recombinant human CCL8. There is no detectable cross-reactivity with other relevant proteins.\

Standard: Expression system for standard: E.coli, Immunogen sequence:  Q24-P99

Anwendungsinformationen

Applikationshinweise:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human CCL8 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human CCL8 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 15.6pg/mL-1000pg/mL

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Details zu CCL8

Target: CCL8 (Chemokine (C-C Motif) Ligand 8 (CCL8))

Andere Bezeichnung: CCL8 - MCP-2 (CCL8 Produkte)

Synonyme: HC14 ELISA Kit, MCP-2 ELISA Kit, MCP2 ELISA Kit, SCYA10 ELISA Kit, SCYA8 ELISA Kit, 1810063B20Rik ELISA Kit, AB023418 ELISA Kit, Mcp2 ELISA Kit, Scya8 ELISA Kit, C-C motif chemokine ligand 8 ELISA Kit, chemokine (C-C motif) ligand 8 ELISA Kit, C-C motif chemokine 8 ELISA Kit, monocyte chemoattractant protein-2 precursor ELISA Kit, CCL8 ELISA Kit, Ccl8 ELISA Kit, LOC101119572 ELISA Kit, MCP-2 ELISA Kit

Gen-ID: 6355

NCBI Accession: NP_005614

UniProt: P80075