CXCL13 ELISA Kit

Produktnummer ABIN6720058

Hersteller Produkt- Nr.: koa0433

Produktdetails CXCL13 ELISA Kit

Target: CXCL13 (Chemokine (C-X-C Motif) Ligand 13 (CXCL13))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.6 pg/mL - 1000 pg/mL

Untere Nachweisgrenze: 15.6 pg/mL

Applikation: ELISA

Hersteller Produkt- Nr.: koa0433

Hersteller: Rockland

Verwendungszweck: Mouse CXCL13 - BLC Sandwich ELISA Kit for Quantitative Detection

Marke: AccuSignal™

Proben: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytische Methode: Quantitative

Spezifität:

Production: Natural and recombinant mouse CXCL13. There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 10 pg/mL

Bestandteile:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Anwendungsinformationen

Applikationshinweise:

ELISA_Dilution: 15.6pg/mL-1000pg/mL

Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL mouse CXCL13 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse CXCL13 standard solution and each sample be measured in duplicate.

Kommentare:

Standard: Expression system for standard: E.coli, Immunogen sequence: I22-A109

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu CXCL13

Target: CXCL13 (Chemokine (C-X-C Motif) Ligand 13 (CXCL13))

Andere Bezeichnung: CXCL13/BLC (CXCL13 Produkte)

Hintergrund:

Synonyms: ANGIE, ANGIE2, B cell attracting chemokine 1, B cell chemoattractant, B cell homing chemokine (ligand for Burkitt's lymphoma receptor 1), B lymphocyte chemoattractant, BCA 1, BLC, BLR1L, C X C motif chemokine 13, Chemokine (C X C motif) ligand 13, Chemokine CXC motif ligand 13, CXC chemokine BLC, CXCL13, SCYB13, Small inducible cytokine B subfamily (Cys X Cys motif) member 13 (B cell chemoattractant), Small inducible cytokine B13, Small inducible cytokine subfamily B member 13

Background: C-X-C motif chemokine 13(CXCL13), also known as B lymphocyte chemoattractant(BLC), is a protein that in humans is encoded by theCXCL13 gene. CXCL13 is a small cytokine belonging to the CXC chemokine family, and it was located on sequence maps to 4q21, near the genes encoding most CXC chemokines. In T-lymphocytes, CXCL13 expression is thought to reflect a germinal center origin of the T-cell. Hence, expression of CXCL13 in T-cell lymphomas, such as Angioimmunoblastic T-cell Lymphoma, is thought to reflect a germinal center origin of the neoplastic T-cells.

Gen-ID: 55985

NCBI Accession: NP_061354

UniProt: O55038