SLAMF1 ELISA Kit
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- Target Alle SLAMF1 ELISA Kits anzeigen
- SLAMF1 (Signaling Lymphocytic Activation Molecule Family Member 1 (SLAMF1))
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Reaktivität
- Human
- Nachweismethode
- Colorimetric
- Methodentyp
- Sandwich ELISA
- Detektionsbereich
- 156 pg/mL - 10000 pg/mL
- Untere Nachweisgrenze
- 156 pg/mL
- Applikation
- ELISA
- Hersteller Produkt- Nr.
- koa0793
- Hersteller
- Rockland
- Verwendungszweck
- Human SLAM - CD150 Sandwich ELISA Kit for Quantitative Detection
- Marke
- AccuSignal™
- Proben
- Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
- Analytische Methode
- Quantitative
- Spezifität
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Production: Natural and recombinant human SLAM. There is no detectable cross-reactivity with other relevant proteins.
- Sensitivität
- < 10 pg/mL
- Bestandteile
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
- Top Product
- Discover our top product SLAMF1 ELISA Kit
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- Applikationshinweise
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human SLAM standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human SLAM standard solution and each sample be measured in duplicate.
- Kommentare
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Standard: Expression system for standard: NSO, Immunogen sequence: A21-K236
- Probenmenge
- 100 μL
- Plattentyp
- Pre-coated
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
- Haltbarkeit
- 12 months
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- Target Alle SLAMF1 ELISA Kits anzeigen
- SLAMF1 (Signaling Lymphocytic Activation Molecule Family Member 1 (SLAMF1))
- Andere Bezeichnung
- SLAM/CD150 (SLAMF1 Produkte)
- Hintergrund
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Synonyms: CD 150, Cd150, CD150 antigen, Cdw150, Estm51, Ipo 3, IPO-3, Ipo3, Signaling lymphocytic activation molecule, Signaling lymphocytic activation molecule family member 1, Signaling lymphocytic activation molecule family member 1 isoform CRA_a, Signaling lymphocytic activation molecule family member 1 isoform CRA_b, Signaling lymphocytic activation molecule precursor, Slaf1, SLAF1_HUMAN, SLAM family member 1, Slamf 1, Slamf1, SLAMF1 protein
Background: Signaling lymphocytic activation molecule is a protein that in humans is encoded by the SLAMF1 gene. It belongs to the immunoglobulin gene superfamily. This gene is mapped to 1q23.3. It has found that SLAM is constitutively expressed on peripheral blood memory T cells, T-cell clones, immature thymocytes and a proportion of B cells, and is rapidly induced on naive T cells after activation. In MV-resistant cell lines, infection with clinical MV and expression of SLAM, but not CD46, caused cytopathic effects (CPE). The expression of SLAM on activated B and T lymphocytes correlates with the pathology of MV infection in humans and monkeys, in which lymphoid organs are the chief sites of MV replication and the binding of MV to SLAM may impair the signaling functions of SLAM in lymphocyte activation and inhibit Th0/Th1 cytokine production, thereby promoting Th2 cytokine production. It has reported that antibody-mediated ligation of SLAM on thymocytes triggered a protein tyrosine phosphorylation signal in T cells in a SAP-dependent manner. This signal also involved SHIP, the adaptor molecules DOK2, DOK1, and SHC and RASGAP.
- Gen-ID
- 6504
- NCBI Accession
- NP_003028
- UniProt
- Q13291
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