Cathepsin D ELISA Kit
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- Target Alle Cathepsin D (CTSD) ELISA Kits anzeigen
- Cathepsin D (CTSD)
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Reaktivität
- Human
- Nachweismethode
- Colorimetric
- Methodentyp
- Sandwich ELISA
- Detektionsbereich
- 156 pg/mL - 10000 pg/mL
- Untere Nachweisgrenze
- 156 pg/mL
- Applikation
- ELISA
- Hersteller Produkt- Nr.
- koa0387
- Hersteller
- Rockland
- Verwendungszweck
- Human Cathepsin D Sandwich ELISA Kit for Quantitative Detection
- Marke
- AccuSignal™
- Proben
- Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
- Analytische Methode
- Quantitative
- Spezifität
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Production: Natural and recombinant human Cathepsin D. There is no detectable cross-reactivity with other relevant proteins.
- Sensitivität
- < 10 pg/mL
- Bestandteile
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
- Top Product
- Discover our top product CTSD ELISA Kit
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- Applikationshinweise
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human Cathepsin D standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human Cathepsin D standard solution and each sample be measured in duplicate.
- Kommentare
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Standard: Expression system for standard: NSO, Immunogen sequence: L21-L412
- Probenmenge
- 100 μL
- Plattentyp
- Pre-coated
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
- Haltbarkeit
- 12 months
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- Target Alle Cathepsin D (CTSD) ELISA Kits anzeigen
- Cathepsin D (CTSD)
- Andere Bezeichnung
- Cathepsin D (CTSD Produkte)
- Hintergrund
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Synonyms: CATD_HUMAN, Cathepsin D heavy chain, Ceroid lipofuscinosis neuronal 10, CLN10, CPSD, ctsd, Lysosomal aspartyl protease
Background: Cathepsin D is a protein that in humans is encoded by the CTSD gene. This proteinase is a member of the peptidase C1 family, having a specificity similar to but narrower than that of pepsin A. It is mapped to 11p15.5. The cDNA encodes a 412-amino acid protein with 20 and 44 amino acids in a pre- and prosegment, respectively. Cathepsin D is one of the lysosomal proteinases. It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. Mutations in this gene are involved in the pathogenesis of several diseases, including breast cancer and possibly Alzheimer disease and it has been considered as a breast cancer tumor marker.
- Gen-ID
- 1509
- NCBI Accession
- NP_001900
- UniProt
- P07339
- Pathways
- Peptide Hormone Metabolism
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