KIT ELISA Kit

Produktnummer ABIN6720112

Produktdetails KIT ELISA Kit

Target: KIT (Mast/stem Cell Growth Factor Receptor (KIT))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 156 pg/mL - 10000 pg/mL

Untere Nachweisgrenze: 156 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich ELISA for Quantitative Detection of Antigen

Proben: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytische Methode: Quantitative

Produktmerkmale:

Synonyms: CD117, CD117 antigen, EC 2.7.10.1, Mast/stem cell growth factor receptor precursor, PBT, Proto oncogene tyrosine protein kinase Kit, SCFR, v kit Hardy Zuckerman 4 feline sarcoma viral oncogene homolog

Background: SCFR(Mast/stem cell growth factor receptor), also known as proto-oncogene c-Kit or tyrosine-protein kinase Kit or CD117, is a protein that in humans is encoded by the KIT gene. KIT was first described as the cellular homolog of the feline sarcoma viral oncogene v-kit. The KIT gene is mapped on 4q12. Kit was expressed on the surface of germ cells up to the pachytene stage. Signaling from the KIT receptor tyrosine kinase is essential for primordial germ cell growth both in vivo and in vitro. Determination of the KIT effectors acting in primordial germ cells has been hampered by the lack of effective methods to manipulate easily gene expression in these cells.

Gene Name: KIT

Production: Natural and recombinant human KIT. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: sf21, Immunogen sequence: Q26-T520

Anwendungsinformationen

Applikationshinweise:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 313pg/mL, 156pg/mL human KIT standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human KIT standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 156pg/mL-10000pg/mL

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Details zu KIT

Target: KIT (Mast/stem Cell Growth Factor Receptor (KIT))

Andere Bezeichnung: KIT - SCFR (KIT Produkte)

Synonyme: C-Kit ELISA Kit, CD117 ELISA Kit, PBT ELISA Kit, SCFR ELISA Kit, Bs ELISA Kit, Fdc ELISA Kit, Gsfsco1 ELISA Kit, Gsfsco5 ELISA Kit, Gsfsow3 ELISA Kit, SCO1 ELISA Kit, SCO5 ELISA Kit, SOW3 ELISA Kit, Ssm ELISA Kit, Tr-kit ELISA Kit, W ELISA Kit, c-KIT ELISA Kit, c-kit ELISA Kit, MGF ELISA Kit, KIT ELISA Kit, C-KIT ELISA Kit, kit ELISA Kit, receptor ELISA Kit, sparse ELISA Kit, Xkl-1 ELISA Kit, kit-a ELISA Kit, kit-b ELISA Kit, kl1-A ELISA Kit, krk1 ELISA Kit, pbt ELISA Kit, scfr ELISA Kit, KIT proto-oncogene receptor tyrosine kinase ELISA Kit, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog a ELISA Kit, KIT proto-oncogene receptor tyrosine kinase S homeolog ELISA Kit, KIT ELISA Kit, Kit ELISA Kit, kita ELISA Kit, kit ELISA Kit, kit.S ELISA Kit

Gen-ID: 3815

NCBI Accession: NP_000213

UniProt: P10721

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Sensory Perception of Sound, Stem Cell Maintenance, Production of Molecular Mediator of Immune Response, Regulation of long-term Neuronal Synaptic Plasticity