VCAM1 ELISA Kit
-
- Target Alle VCAM1 ELISA Kits anzeigen
- VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))
-
Reaktivität
- Human
- Nachweismethode
- Colorimetric
- Methodentyp
- Sandwich ELISA
- Detektionsbereich
- 156 pg/mL - 10000 pg/mL
- Untere Nachweisgrenze
- 156 pg/mL
- Applikation
- ELISA
- Hersteller Produkt- Nr.
- koa0327
- Hersteller
- Rockland
- Verwendungszweck
- Human VCAM-1 Sandwich ELISA Kit for Quantitative Detection
- Marke
- AccuSignal™
- Proben
- Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
- Analytische Methode
- Quantitative
- Spezifität
-
Production: Natural and recombinant human VCAM-1. There is no detectable cross-reactivity with other relevant proteins.
- Sensitivität
- < 10 pg/mL
- Bestandteile
-
- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
- Top Product
- Discover our top product VCAM1 ELISA Kit
-
-
- Applikationshinweise
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 313pg/mL, 156pg/mL human VCAM-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. We recommend that each human VCAM-1 standard solution and each sample is measured in duplicate.
- Kommentare
-
Standard: Expression system for standard: NSO, Immunogen sequence: F25 - E698
- Probenmenge
- 100 μL
- Plattentyp
- Pre-coated
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
- Haltbarkeit
- 12 months
-
- Target Alle VCAM1 ELISA Kits anzeigen
- VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))
- Andere Bezeichnung
- VCAM-1 (VCAM1 Produkte)
- Hintergrund
-
Synonyms: CD106, CD106 antigen, DKFZp779G2333, INCAM 100, INCAM-100, INCAM100, L1CAM, MGC99561, V CAM 1, V-CAM 1, Vascular cell adhesion molecule 1, Vascular cell adhesion protein 1, VCAM 1, VCAM-1, VCAM1, VCAM1_HUMAN
Background: Vascular cell adhesion molecule 1(VCAM-1) is a cell surface glycoprotein adhesive for certain blood leukocytes and tumor cells, which is expressed by activated endothelium in a variety of pathologic conditions including atherosclerosis. Increased expression of VCAM1 is associated with a variety of chronic inflammatory conditions, making its expression and function a target for therapeutic intervention. Integrin alpha4beta1(VLA-4) and VCAM-1 facilitate a critical cell-cell adhesion event required for survival of endothelial and mural cells during vascularization. The VCAM1 gene is assigned to the 1p31-32 region of chromosome 1 based on the analysis of human-mouse hybrid cell lines and in situ hybridization. The standard product used in this kit is recombinant human VCAM-1, consisting of Phe25-Glu698 amino acid sequence of a single chain with the molecular mass of 74KDa. As a result of glycosylation, the molecular mass of 90-95KDa is revealed by SDS-PAGE.
- Gen-ID
- 7412
- NCBI Accession
- NP_001069
- UniProt
- P19320
- Pathways
- Carbohydrate Homeostasis
-