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LGALS1/Galectin 1 ELISA Kit

Rockland koa0449 LGALS1 Reaktivität: Maus Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Rockland
Produktnummer ABIN6720130
Hersteller Produkt- Nr.: koa0449
  • Target Alle LGALS1/Galectin 1 (LGALS1) ELISA Kits anzeigen
    LGALS1/Galectin 1 (LGALS1) (Lectin, Galactoside-Binding, Soluble, 1 (LGALS1))
    Reaktivität
    • 16
    • 13
    • 6
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Maus
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    156 pg/mL - 10000 pg/mL
    Untere Nachweisgrenze
    156 pg/mL
    Applikation
    ELISA
    Hersteller Produkt- Nr.
    koa0449
    Hersteller
    Rockland
    Verwendungszweck
    Mouse Galectin-1 Sandwich ELISA Kit for Quantitative Detection
    Marke
    AccuSignal™
    Proben
    Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
    Analytische Methode
    Quantitative
    Spezifität

    Production: Natural and recombinant mouse Galectin-1. There is no detectable cross-reactivity with other relevant proteins.

    Sensitivität
    < 5 pg/mL
    Bestandteile
    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
    Top Product
    Discover our top product LGALS1 ELISA Kit
  • Applikationshinweise
    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse Galectin-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse Galectin-1 standard solution and each sample be measured in duplicate.
    Kommentare

    Standard: Expression system for standard: E.coli, Immunogen sequence: A2-E135

    Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
    Haltbarkeit
    12 months
  • Target Alle LGALS1/Galectin 1 (LGALS1) ELISA Kits anzeigen
    LGALS1/Galectin 1 (LGALS1) (Lectin, Galactoside-Binding, Soluble, 1 (LGALS1))
    Andere Bezeichnung
    Galectin-1 (LGALS1 Produkte)
    Hintergrund

    Synonyms: 14 kDa laminin-binding protein, 14 kDa lectin, Beta galactoside binding protein 14 kDa, Beta-galactoside-binding lectin L-14-I, Gal-1, GAL1, Galaptin, Galectin-1, GBP, HBL, HLBP14, HPL Lactose-binding lectin 1, Lectin galactoside-binding soluble 1, LEG1_HUMAN, LGALS1, Putative MAPK-activating protein PM12, S-Lac lectin 1

    Background: Galectin-1 is a protein that in humans is encoded by the LGALS1 gene. The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. LGALS1 may act as an autocrine negative growth factor that regulates cell proliferation. Baldini et al. stated that the mouse beta-galactoside-binding protein is an autocrine regulator of cell proliferation with a role in the maintenance of G0 and in the control of G2 traverse. They found that galectin-1 was expressed in a subset of slowly dividing subventricular zone astrocytes, which included the neural stem cells. Intraventricular infusion experiments and phenotypic analysis of knockout mice showed that galectin-1 was an endogenous factor that promoted the proliferation of neural stem cells in adult mouse brain. The standard product used in this kit is recombinant mouse GALECTIN-1, Ala2-Glu135, with the molecular mass of 15KDa.

    Gen-ID
    16852
    NCBI Accession
    NP_032521
    UniProt
    P16045
    Pathways
    Carbohydrate Homeostasis
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