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CD73 ELISA Kit

Rockland koa0859 NT5E Reaktivität: Human Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Rockland
Produktnummer ABIN6720156
Hersteller Produkt- Nr.: koa0859
  • Target Alle CD73 (NT5E) ELISA Kits anzeigen
    CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))
    Reaktivität
    • 8
    • 6
    • 6
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    31.2 pg/mL - 2000 pg/mL
    Untere Nachweisgrenze
    31.2 pg/mL
    Applikation
    ELISA
    Hersteller Produkt- Nr.
    koa0859
    Hersteller
    Rockland
    Verwendungszweck
    Human CD73 - NT5E Sandwich ELISA Kit for Quantitative Detection
    Marke
    AccuSignal™
    Proben
    Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
    Analytische Methode
    Quantitative
    Spezifität

    Production: Natural and recombinant human CD73. There is no detectable cross-reactivity with other relevant proteins.

    Sensitivität
    < 10 pg/mL
    Bestandteile
    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
    Top Product
    Discover our top product NT5E ELISA Kit
  • Applikationshinweise
    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human CD73 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum and plasma(heparin, EDTA) to each empty well. It is recommended that each human CD73 standard solution and each sample be measured in duplicate.
    Kommentare

    Standard: Expression system for standard: CHO, Immunogen sequence: W27-K547

    Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
    Haltbarkeit
    12 months
  • Target Alle CD73 (NT5E) ELISA Kits anzeigen
    CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))
    Andere Bezeichnung
    CD73/NT5E (NT5E Produkte)
    Hintergrund

    Synonyms: 5' NT, 5' nucleotidase (CD73)|5' nucleotidase precursor, 5' nucleotidase ecto, 5' nucleotidase ecto (CD73), 5'-NT, 5'-nucleotidase, 5NTD_HUMAN, CD73, CD73 antigen, E5NT, Ecto 5' nucleotidase, Ecto-5'-nucleotidase, eN, eNT, NT, NT5, NT5E, NTE, Purine 5 Prime Nucleotidase

    Background: 5'-nucleotidase (5'-NT), also known as ecto-5'-nucleotidase or CD73 (Cluster of Differentiation 73), is an enzyme that in humans is encoded by the NT5E gene. Ecto-5-prime-nucleotidase catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. And the enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5 occurs in a variety of immunodeficiency diseases. Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate. Rareallelic variants are associated with a syndrome of adult-onset calcification of joints and arteries (CALJA) affecting the iliac, femoral, and tibial arteries reducing circulation in the legs and the joints of the hands and feet causing pain.

    Gen-ID
    4907
    NCBI Accession
    NP_001191742
    UniProt
    P21589
    Pathways
    Synaptic Membrane, Ribonucleoside Biosynthetic Process
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