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AXL ELISA Kit

Rockland koa0377 AXL Reaktivität: Maus Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Rockland
Produktnummer ABIN6720183
Hersteller Produkt- Nr.: koa0377
  • Target Alle AXL ELISA Kits anzeigen
    AXL (AXL Receptor tyrosine Kinase (AXL))
    Reaktivität
    • 14
    • 5
    • 3
    Maus
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    31.2 pg/mL - 2000 pg/mL
    Untere Nachweisgrenze
    31.2 pg/mL
    Applikation
    ELISA
    Hersteller Produkt- Nr.
    koa0377
    Hersteller
    Rockland
    Verwendungszweck
    Mouse AXL Sandwich ELISA Kit for Quantitative Detection
    Marke
    AccuSignal™
    Proben
    Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
    Analytische Methode
    Quantitative
    Spezifität

    Production: Natural and recombinant mouse AXL. There is no detectable cross-reactivity with other relevant proteins.

    Sensitivität
    < 10 pg/mL
    Bestandteile
    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
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  • Applikationshinweise
    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse AXL standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse AXL standard solution and each sample be measured in duplicate.
    Kommentare

    Standard: Expression system for standard: NSO, Immunogen sequence: H20-P443

    Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
    Haltbarkeit
    12 months
  • Target Alle AXL ELISA Kits anzeigen
    AXL (AXL Receptor tyrosine Kinase (AXL))
    Andere Bezeichnung
    AXL (AXL Produkte)
    Hintergrund

    Synonyms: Adhesion related kinase, AI323647, Ark, AXL oncogene, AXL receptor tyrosine kinase, AXL transforming gene, AXL transforming sequence/gene, JTK11, Oncogene AXL, Tyro7, Tyrosine protein kinase receptor UFO, UFO

    Background: Tyrosine-protein kinase receptor UFO is an enzyme that in humans is encoded by the AXL gene. The protein encoded by this gene is a member of the receptor tyrosine kinase subfamily. Although it is similar to other receptor tyrosine kinases, the Axl protein represents a unique structure of the extracellular region that juxtaposes IgL and FNIII repeats. It transduces signals from the extracellular matrix into the cytoplasm by binding growth factors like vitamin K-dependent protein growth-arrest-specific gene 6. It is involved in the stimulation of cell proliferation. This receptor can also mediate cell aggregation by homophilic binding. Axl is a chronic myelogenous leukemia-associated oncogene and also associated with colon cancer and melanoma. It is in close vicinity to the bcl3 oncogene, which is at 19q13.1-q13.2. The Axl gene is evolutionarily conserved between vertebrate species. This gene has two different alternatively spliced transcript variants.

    Gen-ID
    26362
    NCBI Accession
    NP_033491
    UniProt
    Q00993
    Pathways
    RTK Signalweg, Cellular Response to Molecule of Bacterial Origin
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