Ccl12 ELISA Kit

Produktnummer ABIN6720185

Produktdetails Ccl12 ELISA Kit

Target: Ccl12 (Chemokine (C-C Motif) Ligand 12 (Ccl12))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 31.2 pg/mL - 2000 pg/mL

Untere Nachweisgrenze: 31.2 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich ELISA for Quantitative Detection of Antigen

Proben: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytische Methode: Quantitative

Produktmerkmale:

Synonyms: C C motif chemokine 12, Ccl12, MCP 1 related chemokine, Monocyte chemoattractant protein 5, Monocyte chemotactic protein 5, Scya12, Small inducible cytokine A12

Background: Chemokine(C-C motif) ligand 12(CCL12) is a small cytokine belonging to the CC chemokine family that has been described in mice. It is also known as monocyte chemotactic protein 5(MCP-5) and, due to its similarity with the human chemokine MCP-1, sometimes it is called MCP-1-related chemokine. CCL12 specifically attracts eosinophils, monocytes and lymphocytes. This chemokine is found predominately in lymph nodes and thymus under normal conditions, and its expression can be hugely induced in macrophages. It is thought to coordinate cell movements during early allergic reactions, and immune response to pathogens. The gene for CCL12 is found in a cluster of CC chemokines on mouse chromosome 11.

Gene Name: Ccl12

Production: Natural and recombinant mouse CCL12. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: E.coli, Immunogen sequence: G23-G104

Anwendungsinformationen

Applikationshinweise:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse CCL12 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse CCL12 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 31.2pg/mL-2000pg/mL

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Details zu Ccl12

Target: Ccl12 (Chemokine (C-C Motif) Ligand 12 (Ccl12))

Andere Bezeichnung: CCL12 - MCP5 (Ccl12 Produkte)

Synonyme: MCP-5 ELISA Kit, Scya12 ELISA Kit, chemokine (C-C motif) ligand 12 ELISA Kit, Ccl12 ELISA Kit

Gen-ID: 105242482

NCBI Accession: NP_035461

UniProt: Q62401

Pathways: Cellular Response to Molecule of Bacterial Origin