F11R ELISA Kit

Produktnummer ABIN6720201

Hersteller Produkt- Nr.: koa0721

Produktdetails F11R ELISA Kit

Target: F11R (F11 Receptor (F11R))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 31.2 pg/mL - 2000 pg/mL

Untere Nachweisgrenze: 31.2 pg/mL

Applikation: ELISA

Hersteller Produkt- Nr.: koa0721

Hersteller: Rockland

Verwendungszweck: Mouse JAM A - F11R Sandwich ELISA Kit for Quantitative Detection

Marke: AccuSignal™

Proben: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytische Methode: Quantitative

Spezifität:

Production: Natural and recombinant mouse JAM-A. There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 10 pg/mL

Bestandteile:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Anwendungsinformationen

Applikationshinweise: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse JAM-A standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse JAM-A standard solution and each sample be measured in duplicate.

Kommentare:

Standard: Expression system for standard: NSO, Immunogen sequence: K27-G238

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu F11R

Target: F11R (F11 Receptor (F11R))

Andere Bezeichnung: JAM-A/F11R (F11R Produkte)

Hintergrund:

Synonyms: CD 321, CD321, CD321 antigen, ESTM33, F11 receptor, F11R, JAM 1, JAM A, JAM, JAM-1, JAM-A, JAM1, JAM1_HUMAN, JAMA, JCAM, Jcam1, Junction adhesion molecule 1, Junction adhesion molecule , mouse, homolog of, Junctional adhesion molecule 1, Junctional adhesion molecule A, KAT, Ly106, PAM 1, PAM-1, PAM1, Platelet adhesion molecule 1, Platelet adhesion molecule, Platelet F11 receptor, PRO301, UNQ264, UNQ264/PRO301

Background: Junctional adhesion molecule A(JAM-A) is a protein that in humans is encoded by the F11R gene. It is mapped to 1q23.3. This gene is an immunoglobulin-like molecule that colocalizes with tight junctions in endothelium and epithelium and is also found on blood leukocytes and platelets. JAM-A plays an important role in the regulation of tight junction assembly in epithelia. In addition, it can act as a receptor for reovirus, a ligand for the integrin LFA1, involved in leukocyte transmigration, and a platelet receptor. JAM-A has a nonredundant role in controlling DC motility, trafficking to lymph nodes, and activation of specific immunity.

Gen-ID: 16456

NCBI Accession: NP_766235

UniProt: O88792

Pathways: Cell-Cell Junction Organization