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IGFBP4 ELISA Kit

Rockland koa0205 IGFBP4 Reaktivität: Human Colorimetric Sandwich ELISA 500 pg/mL - 32000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Rockland
Produktnummer ABIN6720208
Hersteller Produkt- Nr.: koa0205
  • Target Alle IGFBP4 ELISA Kits anzeigen
    IGFBP4 (Insulin-Like Growth Factor Binding Protein 4 (IGFBP4))
    Reaktivität
    • 12
    • 8
    • 6
    • 5
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    500 pg/mL - 32000 pg/mL
    Untere Nachweisgrenze
    500 pg/mL
    Applikation
    ELISA
    Hersteller Produkt- Nr.
    koa0205
    Hersteller
    Rockland
    Verwendungszweck
    Human IGFBP4 Sandwich ELISA Kit for Quantitative Detection
    Marke
    AccuSignal™
    Proben
    Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
    Analytische Methode
    Quantitative
    Spezifität

    Production: Natural and recombinant human IGFBP4. There is no detectable cross-reactivity with other relevant proteins.

    Sensitivität
    < 20 pg/mL
    Bestandteile
    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
    Top Product
    Discover our top product IGFBP4 ELISA Kit
  • Applikationshinweise
    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 32000pg/mL, 16000pg/mL, 8000pg/mL, 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL human IGFBP4 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human IGFBP4 standard solution and each sample be measured in duplicate.
    Kommentare

    Standard: Expression system for standard: NSO, Immunogen sequence: D22-E258

    Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
    Haltbarkeit
    12 months
  • Target Alle IGFBP4 ELISA Kits anzeigen
    IGFBP4 (Insulin-Like Growth Factor Binding Protein 4 (IGFBP4))
    Andere Bezeichnung
    IGFBP4 (IGFBP4 Produkte)
    Hintergrund

    Synonyms: AI875747, BP 4, BP4, Deb2, HT29 IGFBP, IBP 4, IBP-4, IBP4, IBP4_HUMAN, IGF binding protein 4, IGF-binding protein 4, IGF-BP4, IGFBP 4, IGFBP-4, IGFBP4, Insulin Like Growth Factor Binding Protein 4, Insulin-like growth factor-binding protein 4

    Background: Insulin-like growth factor-binding protein 4 is a protein that in humans is encoded by the IGFBP4gene. This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma in both glycosylated and non-glycosylated forms. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. In addition, IGFBP-4 is a unique protein and it consistently inhibits several cancer cells in vivo and in vitro. Its inhibitory action has been shown in vivo in prostate and colon. It is secreted by all colon cancer cells.

    Gen-ID
    3487
    NCBI Accession
    NP_001543
    UniProt
    P22692
    Pathways
    WNT Signalweg, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process
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