Azurocidin ELISA Kit

Produktnummer ABIN6720212

Produktdetails Azurocidin ELISA Kit

Target: Azurocidin (AZU1) (Azurocidin 1 (AZU1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 62.5 pg/mL - 4000 pg/mL

Untere Nachweisgrenze: 62.5 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich ELISA for Quantitative Detection of Antigen

Proben: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytische Methode: Quantitative

Produktmerkmale:

Synonyms: Azu 1, AZU1, Azurocidin, CAP37, CAP7_HUMAN, Cationic antimicrobial protein 37, Cationic antimicrobial protein CAP37, HBP, Heparin binding protein, Heparin-binding protein, HUMAZUR, Neutrophil azurocidin

Background: Azurocidin, also known as cationic antimicrobial protein CAP37 or heparin-binding protein (HBP), is a protein that in humans is encoded by the AZU1 gene. This encoded protein is a member of the serine protease gene family, but it is not a serine proteinase, because the active site serine and histidine residues are replaced. Azurocidin is mapped to 19p13.3. The protein encoded by this gene is an azurophil granule antibiotic protein, with antibacterial activity. It is also an important multifunctional inflammatory mediator. In addition to it, Azurocidin is also a specific chemoattractant for monocytes. It lacks the chemotactic activity for neutrophils and lymphocytes, and this gene is probably responsible for the wave of monocytes that follows the initial wave of PMNs typical of the inflammatory response.

Gene Name: AZU1

Production: Natural and recombinant human Azurocidin. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: I27-P248

Anwendungsinformationen

Applikationshinweise:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human Azurocidin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human Azurocidin standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 62.5pg/mL-4000pg/mL

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Details zu Azurocidin

Target: Azurocidin (AZU1) (Azurocidin 1 (AZU1))

Andere Bezeichnung: Azurocidin (AZU1 Produkte)

Synonyme: AZAMP ELISA Kit, AZU ELISA Kit, CAP37 ELISA Kit, HBP ELISA Kit, HUMAZUR ELISA Kit, NAZC ELISA Kit, AZU1 ELISA Kit, Azurocidin ELISA Kit, pHBP ELISA Kit, azurocidin 1 ELISA Kit, azurocidin ELISA Kit, AZU1 ELISA Kit, CpipJ_CPIJ007384 ELISA Kit

Gen-ID: 566

NCBI Accession: NP_001691

UniProt: P20160