Cathepsin L ELISA Kit

Produktnummer ABIN6720215

Produktdetails Cathepsin L ELISA Kit

Target: Cathepsin L (CTSL1) (Cathepsin L1 (CTSL1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 62.5 pg/mL - 4000 pg/mL

Untere Nachweisgrenze: 62.5 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich ELISA for Quantitative Detection of Antigen

Proben: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytische Methode: Quantitative

Produktmerkmale:

Synonyms: Cathepsin L1, Cathepsin L1 light chain, CathepsinL, CATL, CATL1_HUMAN, CTSL, CTSL1, FLJ31037, Major excreted protein, MEP

Background: Cathepsin L is an important lysosomal endopeptidase enzyme which is involved in the initiation of protein degradation. It also shows the most potent collagenolytic and elastinolytic activity in vitro of any of the cathepsins. The gene is mapped to 9q21-q22. It is a member of the Peptidase C1 family, which play an important role in diverse processes including normal lysosome mediated protein turnover, antigen and proprotein processing, and apoptosis. Cathepsin L has been reported in many organisms including fish, birds and mammals.

Gene Name: CTSL

Production: Natural and recombinant human Cathepsin L. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: E113-V333

Anwendungsinformationen

Applikationshinweise:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human Cathepsin L standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human Cathepsin L standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 62.5pg/mL-4000pg/mL

Other Performance Data: DOE: 2-8° C - 04/25/18 -20° C - 10/26/18

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Details zu Cathepsin L

Target: Cathepsin L (CTSL1) (Cathepsin L1 (CTSL1))

Andere Bezeichnung: Cathepsin L (CTSL1 Produkte)

Synonyme: CATL ELISA Kit, CTSL1 ELISA Kit, MEP ELISA Kit, 1190035F06Rik ELISA Kit, Ctsl1 ELISA Kit, fs ELISA Kit, nkt ELISA Kit, CATHL ELISA Kit, CatL ELISA Kit, Ctsl ELISA Kit, CTSL ELISA Kit, mep ELISA Kit, catl ELISA Kit, ctsl ELISA Kit, cpl-1 ELISA Kit, MGC80629 ELISA Kit, MGC107932 ELISA Kit, LOC663234 ELISA Kit, 26-29-p ELISA Kit, Cp1 ELISA Kit, si:dkey-121a11.6 ELISA Kit, CTSV ELISA Kit, catL ELISA Kit, cb15 ELISA Kit, ctsl1b ELISA Kit, hgg1 ELISA Kit, wu:fb30g09 ELISA Kit, zgc:92089 ELISA Kit, cathepsin L ELISA Kit, cathepsin V ELISA Kit, cathepsin L L homeolog ELISA Kit, cathepsin L1 ELISA Kit, Cathepsin L1 ELISA Kit, Cathepsin L ELISA Kit, testin gene ELISA Kit, cathepsin 12 ELISA Kit, cathepsin Lb ELISA Kit, cathepsin L.1 ELISA Kit, CTSL ELISA Kit, Ctsl ELISA Kit, CTSV ELISA Kit, ctsl.L ELISA Kit, ctsl ELISA Kit, LOC100008830 ELISA Kit, catl1 ELISA Kit, 26-29-p ELISA Kit, catl ELISA Kit, Tsp_11596 ELISA Kit, Testin ELISA Kit, cts12 ELISA Kit, LOC102156614 ELISA Kit, ctslb ELISA Kit, ctsl.1 ELISA Kit

Gen-ID: 1514

NCBI Accession: NP_001244900

UniProt: P07711

Pathways: Activation of Innate immune Response, Toll-Like Receptors Cascades