TGFBI ELISA Kit

Produktnummer ABIN6720234

Produktdetails TGFBI ELISA Kit

Target: TGFBI (Transforming Growth Factor, beta-Induced, 68kDa (TGFBI))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 62.5 pg/mL - 4000 pg/mL

Untere Nachweisgrenze: 62.5 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich ELISA for Quantitative Detection of Antigen

Proben: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytische Methode: Quantitative

Produktmerkmale:

Synonyms: Beta ig-h3, beta-IG-H3, BIGH3, CDB1, CDG2, CDGG1, CSD, CSD1, CSD2, CSD3, EBMD, Kerato-epithelin, LCD1, RGD-CAP, RGD-containing collagen-associated protein, TGFBI, Transforming growth factor-beta-induced protein ig-h3

Background: Transforming growth factor, beta-induced, 68 kDa, also known as TGFBI(initially called BIGH3, BIG-H3), is a protein which in humans is encoded by the TGFBI gene. It is mapped to 5q31.1. This gene encodes an RGD-containing protein that binds to type I, II and IV collagens. The RGD motif is found in many extracellular matrix proteins modulating cell adhesion and serves as a ligand recognition sequence for several integrins. This protein plays a role in cell-collagen interactions and may be involved in endochondrial bone formation in cartilage. The protein is induced by transforming growth factor-beta and acts to inhibit cell adhesion.

Gene Name: TGFBI

Production: Natural and recombinant human TGFBI. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: G24-H683

Anwendungsinformationen

Applikationshinweise:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human TGFBI standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human TGFBI standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 62.5pg/mL-4000pg/mL

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Details zu TGFBI

Target: TGFBI (Transforming Growth Factor, beta-Induced, 68kDa (TGFBI))

Andere Bezeichnung: TGFBI - beta IG-H3 (TGFBI Produkte)

Synonyme: TGFBI ELISA Kit, BIGH3 ELISA Kit, CDB1 ELISA Kit, CDG2 ELISA Kit, CDGG1 ELISA Kit, CSD ELISA Kit, CSD1 ELISA Kit, CSD2 ELISA Kit, CSD3 ELISA Kit, EBMD ELISA Kit, LCD1 ELISA Kit, 68kDa ELISA Kit, AI181842 ELISA Kit, AI747162 ELISA Kit, Beta-ig ELISA Kit, big-h3 ELISA Kit, Big-h3 ELISA Kit, MP70 ELISA Kit, MP78 ELISA Kit, hm:zeh0933 ELISA Kit, wu:fa13f05 ELISA Kit, zgc:110495 ELISA Kit, transforming growth factor beta induced ELISA Kit, transforming growth factor, beta induced ELISA Kit, transforming growth factor, beta-induced ELISA Kit, TGFBI ELISA Kit, Tgfbi ELISA Kit, tgfbi ELISA Kit

Gen-ID: 7045

NCBI Accession: NP_000349

UniProt: Q15582