LTA ELISA Kit

Produktnummer ABIN6720236

Produktdetails LTA ELISA Kit

Target: LTA (Lymphotoxin-alpha (LTA))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 62.5 pg/mL - 4000 pg/mL

Untere Nachweisgrenze: 62.5 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich ELISA for Quantitative Detection of Antigen

Proben: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytische Methode: Quantitative

Produktmerkmale:

Synonyms: Coley's toxin, DAMA-25N12.13-004, DIF, Differentiation inducing factor, Hemorrhagic factor, LT, LT alpha, LT-alpha, Lta, LTalpha, Lymphotoxin alpha, Lymphotoxin alpha (TNF superfamily, member 1), Lymphotoxin-alpha, Natural killer cytotoxic factor, Necrosin, NKCF, TNF B, TNF superfamily member 1, TNF-beta, TNFB, TNFB_HUMAN, TNFbeta, TNFSF 1, TNFSF1, Tumor necrosis factor beta, Tumor necrosis factor ligand superfamily member 1, Tumor necrosis factor superfamily member 1

Background: Tumor necrosis factor-beta(TNF-beta), previously called lymphotoxin-alpha(LTA), is a cytokine produced by lymphocytes. TNF-alpha and TNF-beta share 35 % identity and 50 % homology in the amino acid sequence. The substance LTA mediates a wide variety of inflammatory, immunostimulatory, and antiviral responses. LTA is associated with susceptibility to myocardial infarction, asthma and other diseases. The LTA gene is located on human chromosome 6. The standard product used in this kit is recombinant protein, consisting of 172 amino acids with the molecular mass of 18.8KDa.

Gene Name: LTA

Production: Natural and recombinant human TNF beta. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: E.coli, Immunogen sequence: L35-L205

Anwendungsinformationen

Applikationshinweise:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human TNF beta standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. It is recommended that each human TNF beta standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 62.5pg/mL-4000pg/mL

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Details zu LTA

Target: LTA (Lymphotoxin-alpha (LTA))

Andere Bezeichnung: TNF beta (LTA Produkte)

Synonyme: LT ELISA Kit, TNFB ELISA Kit, TNFSF1 ELISA Kit, LT-[a] ELISA Kit, LT-alpha ELISA Kit, LT[a] ELISA Kit, LTalpha ELISA Kit, Ltx ELISA Kit, TNF-beta ELISA Kit, Tnfb ELISA Kit, Tnfsf1b ELISA Kit, hlb382 ELISA Kit, lymphotoxin alpha ELISA Kit, lymphotoxin A ELISA Kit, LTA ELISA Kit, Lta ELISA Kit

Substanzklasse: Chemical

Gen-ID: 4049

NCBI Accession: NP_000586

UniProt: P01374

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process