IGFBPI ELISA Kit

Produktnummer ABIN6720316

Produktdetails IGFBPI ELISA Kit

Target: IGFBPI (Insulin-Like Growth Factor Binding Protein 1 (IGFBPI))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 31.2 pg/mL - 2000 pg/mL

Untere Nachweisgrenze: 31.2 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich ELISA for Quantitative Detection of Antigen

Proben: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Urine

Analytische Methode: Quantitative

Produktmerkmale:

Synonyms: AFBP, hIGFBP-1, IBP1, IGF-binding protein 1, IGF-BP25, Insulin-like growth factor-binding protein 1, Placental protein 12, PP12

Background: IGFBP1 is also known as amniotic fluid binding protein(AFBP), placental protein-12, alpha-pregnancy-associated endometrial globulin, growth hormone independent binding protein, binding protein-28, binding protein-26, and binding protein-25. The low-molecular weight insulin-like growth-factor binding protein(IGF-BP25) is synthesized by human liver, secretory endometrium and decidua, and is also present in human serum. It binds insulin-like growth factors IGF-I and IGF-II with high affinity, and is proposed to act as a paracrine regulator of cell growth. These IGF-binding proteins are expressed at different concentrations in different tissues and are thought to regulate the activity of IGF I and II. The gene is organized in four exons and spans 5.9 kb. The IBP-1 gene is a single copy gene, located on chromosome 7. The standard product used in this kit is recombinant human IGFBP-1, consisting of 234 amino acids with the molecular mass of 25KDa.

Gene Name: IGFBP1

Production: Natural and recombinant human IGFBP-1. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: A26-N259

Anwendungsinformationen

Applikationshinweise:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human IGFBP-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma (heparin, EDTA) or urine to each empty well. It is recommended that each human IGFBP-1 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 31.2pg/mL-2000pg/mL

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Details zu IGFBPI

Target: IGFBPI (Insulin-Like Growth Factor Binding Protein 1 (IGFBPI))

Andere Bezeichnung: IGFBP-1 (IGFBPI Produkte)

Synonyme: cb656 ELISA Kit, igfbp1 ELISA Kit, IGFBP-1 ELISA Kit, MGC68538 ELISA Kit, afbp ELISA Kit, ibp1 ELISA Kit, pp12 ELISA Kit, igf-bp25 ELISA Kit, higfbp-1 ELISA Kit, IGFBP1 ELISA Kit, LOC100223208 ELISA Kit, LOC100305121 ELISA Kit, AFBP ELISA Kit, IBP1 ELISA Kit, IGF-BP25 ELISA Kit, PP12 ELISA Kit, hIGFBP-1 ELISA Kit, IGFBA ELISA Kit, insulin like growth factor binding protein 1 ELISA Kit, insulin-like growth factor binding protein 1a ELISA Kit, insulin like growth factor binding protein 1 S homeolog ELISA Kit, insulin like growth factor binding protein 1 L homeolog ELISA Kit, insulin-like growth factor binding protein 1 ELISA Kit, IGFBP1 ELISA Kit, igfbp1a ELISA Kit, igfbp1.S ELISA Kit, igfbp1.L ELISA Kit, igfbp1 ELISA Kit, LOC100305121 ELISA Kit, Igfbp1 ELISA Kit

Gen-ID: 3484

NCBI Accession: NP_000587

UniProt: P08833

Pathways: Myometrial Relaxation and Contraction, ER-Nucleus Signaling, Growth Factor Binding