Adipsin ELISA Kit

Produktnummer ABIN6720317

Hersteller Produkt- Nr.: koa0696

Produktdetails Adipsin ELISA Kit

Target: Adipsin (CFD) (Complement Factor D (CFD))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 312 pg/mL - 20000 pg/mL

Untere Nachweisgrenze: 312 pg/mL

Applikation: ELISA

Hersteller Produkt- Nr.: koa0696

Hersteller: Rockland

Verwendungszweck: Human CFD Sandwich ELISA Kit for Quantitative Detection

Marke: AccuSignal™

Proben: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Urine

Analytische Methode: Quantitative

Spezifität:

Production: Natural and recombinant human CFD . There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 10 pg/mL

Bestandteile:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Anwendungsinformationen

Applikationshinweise: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 20,000pg/mL, 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human CFD standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma (heparin, EDTA) or urine to each empty well. We recommend that each human CFD standard solution and each sample is measured in duplicate.

Kommentare:

Standard: Expression system for standard: NSO, Immunogen sequence: I26-A253

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu Adipsin

Target: Adipsin (CFD) (Complement Factor D (CFD))

Andere Bezeichnung: CFD (CFD Produkte)

Hintergrund:

Synonyms: Adipsin, ADN, C3 convertase activator, CFAD_HUMAN, CFD, Complement factor D, Complement factor D preproprotein, D component of complement, DF, PFD, Properdin factor D

Background: Complement factor D(CFD), also called ADIPSIN or FACTOR D, is a protein which in humans is encoded by the CFD gene. The protein encoded by this gene belongs to the trypsin family of peptidases. It is mapped to 19p13.3. The encoded protein is a component of the alternative complement pathway which is best known for its role in humoral suppression of infectious agents. This protein is also a serine protease that is secreted by adipocytes into the bloodstream. Finally, the encoded protein has a high level of expression in fat, suggesting a role for adipose tissue in immune system biology. What's more, Factor D is a serine protease that stimulates glucose transport for triglyceride accumulation in fats cells and inhibits lipolysis.

Gen-ID: 1675

NCBI Accession: NP_001304264

UniProt: P00746

Pathways: Komplementsystem