TIMP4 ELISA Kit

Produktnummer ABIN6720333

Hersteller Produkt- Nr.: koa0316

Produktdetails TIMP4 ELISA Kit

Target: TIMP4 (TIMP Metallopeptidase Inhibitor 4 (TIMP4))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 78 pg/mL - 5000 pg/mL

Untere Nachweisgrenze: 78 pg/mL

Applikation: ELISA

Hersteller Produkt- Nr.: koa0316

Hersteller: Rockland

Verwendungszweck: Human TIMP-4 Sandwich ELISA Kit for Quantitative Detection

Marke: AccuSignal™

Proben: Breast Milk, Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytische Methode: Quantitative

Spezifität:

Production: Natural and recombinant human TIMP-4. This kit detects recombinant human TIMP-4 in both free and MMP-bound forms.

Sensitivität: < 10 pg/mL

Bestandteile:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Anwendungsinformationen

Applikationshinweise: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL, 78pg/mL human TIMP-4 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin , EDTA) or human milk to each empty well. We recommend that each human TIMP-4 standard solution and each sample is measured in duplicate.

Kommentare:

Standard: Expression system for standard: sf21, Immunogen sequence: C30-P224

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu TIMP4

Target: TIMP4 (TIMP Metallopeptidase Inhibitor 4 (TIMP4))

Andere Bezeichnung: TIMP-4 (TIMP4 Produkte)

Hintergrund:

Synonyms: Metalloproteinase inhibitor 4, TIMP 4, TIMP metallopeptidase inhibitor 4, Tissue inhibitor of metalloproteinase 4, Tissue inhibitor of metalloproteinases 4

Background: Tissue inhibitor of metalloproteinases 4, also called Metalloproteinase inhibitor 4, is an enzyme that in humans is encoded by the TIMP4 gene. This gene belongs to the TIMP gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. This gene is mapped to 3p25.2. The secreted, netrin domain-containing protein encoded by this gene is involved in regulation of platelet aggregation and recruitment and may play role in hormonal regulation and endometrial tissue remodeling.

Gen-ID: 7079

NCBI Accession: NP_003247

UniProt: Q99727