TGFA ELISA Kit

Produktnummer ABIN6720341

Produktdetails TGFA ELISA Kit

Target: TGFA (Transforming Growth Factor, alpha (TGFA))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.6 pg/mL - 1000 pg/mL

Untere Nachweisgrenze: 15.6 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich ELISA for Quantitative Detection of Antigen

Proben: Cell Culture Supernatant, Milk, Plasma (EDTA - heparin), Serum

Analytische Methode: Quantitative

Produktmerkmale:

Synonyms: EGF like TGF, ETGF, OTTHUMP00000203300, OTTHUMP00000203301, OTTHUMP00000203345, OTTHUMP00000204192, Protransforming growth factor alpha, RATTGFAA, TFGA, TGF A, TGF type 1, TGFA, Transforming growth factor alpha, Transforming growth factor alpha precursor, Wa1, Waved 1

Background: Transforming growth factor alpha(TGF-alpha) is upregulated in some human cancers. It is produced in macrophages, brain cells, and keratinocytes, and induces epithelial development. It is closely related to EGF, and can also bind to the EGF receptor with similar effects. TGFalpha stimulates neural cell proliferation in the adult injured brain. Transforming growth factor alpha gene(TGFA) maps to human chromosome 2 close to the breakpoint of the t(2,8) variant translocation in Burkitt lymphoma. Synthetic TGF-alpha was as active as murine epidermal growth factor in binding to the epidermal growth factor receptor and in stimulation of anchorage-dependent and of anchorage-independent growth of normal indicator cells in culture. Synthetic TGF-alpha stimulated plasminogen activator production in A 431 and HeLa cells, the stimulation was similar to that induced by epidermal growth factor. Furthermore, synthetic human TGF-alpha showed similar immunoreactivity when compared with rat TGF-alpha. Thus, the 50-amino acid TGF-alpha is likely to be the bioactive principle produced and secreted by tumor cell lines.

Gene Name: TGFA

Production: Natural and recombinant human TGF alpha. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: E.coli, Immunogen sequence: V40-A89

Anwendungsinformationen

Applikationshinweise:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human TGF alpha standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA) or milk to each empty well. It is recommended that each human TGF alpha standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 15.6pg/mL-1000pg/mL

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Details zu TGFA

Target: TGFA (Transforming Growth Factor, alpha (TGFA))

Andere Bezeichnung: TGF alpha (TGFA Produkte)

Synonyme: TFGA ELISA Kit, LOC100009482 ELISA Kit, TGF alpha ELISA Kit, TGFA ELISA Kit, wa-1 ELISA Kit, wa1 ELISA Kit, RATTGFAA ELISA Kit, TGFAA ELISA Kit, NV15606 ELISA Kit, si:ch211-10e8.7 ELISA Kit, transforming growth factor alpha ELISA Kit, transforming growth factor alpha L homeolog ELISA Kit, transforming growth factor-alpha ELISA Kit, transforming growth factor, alpha ELISA Kit, TGFA ELISA Kit, TGF-A ELISA Kit, Tgfa ELISA Kit, tgfa.L ELISA Kit, LOC100121300 ELISA Kit, LOC100009150 ELISA Kit, tgfa ELISA Kit

Gen-ID: 7039

NCBI Accession: NP_001093161

UniProt: P01135

Pathways: NF-kappaB Signalweg, RTK Signalweg, EGFR Signaling Pathway