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MIA ELISA Kit

Rockland koa0576 MIA Reaktivität: Human Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Saliva, Serum
Rockland
Produktnummer ABIN6720344
Hersteller Produkt- Nr.: koa0576
  • Target Alle MIA ELISA Kits anzeigen
    MIA (Melanoma Inhibitory Activity (MIA))
    Reaktivität
    • 7
    • 3
    • 2
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    15.6 pg/mL - 1000 pg/mL
    Untere Nachweisgrenze
    15.6 pg/mL
    Applikation
    ELISA
    Hersteller Produkt- Nr.
    koa0576
    Hersteller
    Rockland
    Verwendungszweck
    Human MIA Sandwich ELISA Kit for Quantitative Detection
    Marke
    AccuSignal™
    Proben
    Cell Culture Supernatant, Plasma (EDTA - heparin), Saliva, Serum
    Analytische Methode
    Quantitative
    Spezifität

    Production: Natural and recombinant human MIA. There is no detectable cross-reactivity with other relevant proteins.

    Sensitivität
    < 10 pg/mL
    Bestandteile
    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
    Top Product
    Discover our top product MIA ELISA Kit
  • Applikationshinweise
    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human MIA standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA) or saliva to each empty well. It is recommended that each human MIA standard solution and each sample be measured in duplicate.
    Kommentare

    Standard: Expression system for standard: E.coli, Immunogen sequence: G25-131Q

    Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
    Haltbarkeit
    12 months
  • Target Alle MIA ELISA Kits anzeigen
    MIA (Melanoma Inhibitory Activity (MIA))
    Andere Bezeichnung
    MIA (MIA Produkte)
    Hintergrund

    Synonyms: CD RAP, melanoma inhibitory activity, Melanoma inhibitory activity protein, Melanoma-derived growth regulatory protein, Mia, MIA_HUMAN

    Background: Melanoma Inhibiting Activity(MIA), also known as cartilage-derived retinoic acid-sensitive protein(CD-RAP), is an approximately 11-15 kDa protein that is expressed as a noncovalent homodimer. MIA is structurally related to OTOR/Otoraplin and MIA-2 in a small family of secreted proteins with one SH3 domain(1-3). And the MIA gene was mapped to 19q13.32-q13.33 by fluorescence in situ hybridization. Beside, It is a marker for malignant melanoma. MIA functions as a chemoattractant for mesenchymal stem cells and enhances their BMP-2 and TGF-beta3 induced differentiation into chondrocytes . MIA-deficient mice exhibit delayed chondrocyte differentiation but enhanced chondrocyte proliferation and cartilage repair .

    Gen-ID
    8190
    NCBI Accession
    NP_001189482
    UniProt
    Q16674
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