Complement Factor H ELISA Kit

Produktnummer ABIN6720349

Hersteller Produkt- Nr.: koa0843

Produktdetails Complement Factor H ELISA Kit

Target: Complement Factor H (CFH)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 1.56 ng/mL - 100 ng/mL

Untere Nachweisgrenze: 1.56 ng/mL

Applikation: ELISA

Hersteller Produkt- Nr.: koa0843

Hersteller: Rockland

Verwendungszweck: Human Complement H - CFH Sandwich ELISA Kit for Quantitative Detection

Marke: AccuSignal™

Proben: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Urine

Analytische Methode: Quantitative

Spezifität:

Production: Natural and recombinant human CFH. There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 50 pg/mL

Bestandteile:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Anwendungsinformationen

Applikationshinweise: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 100 ng/mL, 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL human CFH standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA) or urine to each empty well. It is recommended that each human CFH standard solution and each sample be measured in duplicate.

Kommentare:

Standard: Expression system for standard: NSO, Immunogen sequence: S860-R1231

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu Complement Factor H

Target: Complement Factor H (CFH)

Andere Bezeichnung: Complement H/CFH (CFH Produkte)

Hintergrund:

Synonyms: adrenomedullin binding protein, age related maculopathy susceptibility 1, AHUS 1, AHUS1, AMBP 1, AMBP1, ARMD 4, ARMD4, ARMS 1, ARMS1, beta 1 H globulin, beta 1H, beta1H, CFAH_HUMAN, CFH, CFHL 3, CFHL3, Complement factor H, complement factor H isoform b, Factor H, factor H like 1, FH, FHL 1, FHL1, H factor 1 (complement)|H factor 1, H factor 2 (complement), HF 1, HF 2, HF, HF1, HF2, HUS, MGC88246

Background: Complement factor H (CFH), originally known as beta-1H globulin, is a serum glycoprotein that regulates the function of the alternative complement pathway in fluid phase and on cellular surfaces. It binds to C3b, accelerates the decay of the alternative pathway convertase C3bBb, and also acts as a cofactor for complement factor I, another C3b inhibitor. The CFH gene is located on chromosome 1q32-q32.1 within a cluster of genes encoding the regulatory complement components of the activation of C3 (RCA for 'regulators of complement activation'). This gene cluster includes decay-accelerating factor (DAF), C4-binding protein (C4BPA and C4BPB), and the factor H-related genes CFHR1, CFHR2, CFHR3, CFHR4, and CFHR5, among others. The gene family has arisen by multiple duplication events.

Gen-ID: 3075

NCBI Accession: NP_000177

UniProt: P08603

Pathways: Komplementsystem, Cellular Response to Molecule of Bacterial Origin