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PRSS8 ELISA Kit

Rockland koa0748 PRSS8 Reaktivität: Human Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Urine
Rockland
Produktnummer ABIN6720350
Hersteller Produkt- Nr.: koa0748
  • Target Alle PRSS8 ELISA Kits anzeigen
    PRSS8 (Protease, serine, 8 (PRSS8))
    Reaktivität
    • 12
    • 5
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    1.56 ng/mL - 100 ng/mL
    Untere Nachweisgrenze
    1.56 ng/mL
    Applikation
    ELISA
    Hersteller Produkt- Nr.
    koa0748
    Hersteller
    Rockland
    Verwendungszweck
    Human Prostasin Sandwich ELISA Kit for Quantitative Detection
    Marke
    AccuSignal™
    Proben
    Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Urine
    Analytische Methode
    Quantitative
    Spezifität

    Production: Natural and recombinant human Prostasin. There is no detectable cross-reactivity with other relevant proteins.

    Sensitivität
    < 50 pg/mL
    Bestandteile
    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
    Top Product
    Discover our top product PRSS8 ELISA Kit
  • Applikationshinweise
    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 100 ng/mL, 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL human Prostasin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA) or urine to each empty well. It is recommended that each human Prostasin standard solution and each sample be measured in duplicate.
    Kommentare

    Standard: Expression system for standard: CHO, Immunogen sequence: A33-G319

    Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
    Haltbarkeit
    12 months
  • Target Alle PRSS8 ELISA Kits anzeigen
    PRSS8 (Protease, serine, 8 (PRSS8))
    Andere Bezeichnung
    Prostasin (PRSS8 Produkte)
    Hintergrund

    Synonyms: 2410039E18Rik, AI313909, C79772, CAP1, Channel Activating Protease 1, mCAP1, Prostasin, Prostasin heavy chain, protease, serine, 8, Prss8, PRSS8_HUMAN, Serine protease 8

    Background: Prostasin is a protein that in humans is encoded by the PRSS8 gene. It is a single-copy gene and mapped to 16p11.2. Prostasin is likely to be the more physiologically relevant protease. This gene encodes a trypsinogen, which is a member of the trypsin family of serine proteases. Coexpression of Prostasin with either Xenopus or rat ENaC in Xenopus oocytes resulted in a 60 % to 80 % increase in amiloride-sensitive sodium currents, and that the addition of aprotinin, a serine protease inhibitor, completely prevented this activation. This enzyme is highly expressed in prostate epithelia and is one of several proteolytic enzymes found in seminal fluid. The proprotein is cleaved to produce a light chain and a heavy chain which are associated by a disulfide bond. It is active on peptide linkages involving the carboxyl group of lysine or arginine.

    Gen-ID
    5652
    NCBI Accession
    NP_002764
    UniProt
    Q16651
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