CX3CL1 ELISA Kit

Produktnummer ABIN6720364

Produktdetails CX3CL1 ELISA Kit

Target: CX3CL1 (Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 156 pg/mL - 10000 pg/mL

Untere Nachweisgrenze: 156 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich ELISA for Quantitative Detection of Antigen

Proben: Breast Milk, Cell Culture Supernatant, Plasma (EDTA - heparin), Saliva, Serum, Urine

Analytische Methode: Quantitative

Produktmerkmale:

Synonyms: A 152E5.2, AB030188, ABCD 3, ABCD3, C X3 C motif chemokine 1, C3Xkine, Chemokine (C X3 C motif) ligand 1, Chemokine C X3 C motif ligand 1, Chemokine CX3C Motif Ligand 1, CX3C membrane anchored chemokine, CXC3, CXC3C, FKN, Fractalkine, Neurotactin, NTN, NTT, SCYD 1, SCYD1, Small inducible cytokine D1, Small inducible cytokine subfamily D (Cys X3 Cys), member 1, Small inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine, neurotactin), Small inducible cytokine subfamily D member 1

Background: Chemokine(C-X3-C motif) ligand 1(CX3CL1) is a large cytokine protein of 373 amino acids, it contains multiple domains and is the only known member of the CX3C chemokine family. It is also commonly known under the names fractalkine(in humans) and neurotactin(in mice). CX3CL1 is produced as a long protein(with 373-amino acid in humans) with an extended mucin-like stalk and a chemokine domain on top. The mucin-like stalk permits it to bind to the surface of certain cells. However a soluble(90 kD) version of this chemokine has also been observed. Soluble CX3CL1 potently chemoattracts T cells and monocytes, while the cell-bound chemokine promotes strong adhesion of leukocytes to activated endothelial cells, where it is primarily expressed. CX3CL1 elicits its adhesive and migratory functions by interacting with the chemokine receptor CX3CR1. Its gene is located on human chromosome 16 along with some CC chemokines known as CCL17 and CCL22. It can act as a mediator of smooth muscle cell migration in human atherosclerosis, rather than mediate inflammatory cell recruitment.

Gene Name: CX3CL1

Production: Natural and recombinant human Fractalkine. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: G27-R189

Anwendungsinformationen

Applikationshinweise:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human Fractalkine standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA), saliva, urine or human milk to each empty well. It is recommended that each human Fractalkine standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 156pg/mL-10000pg/mL

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Details zu CX3CL1

Target: CX3CL1 (Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1))

Andere Bezeichnung: Fractalkine - CX3CL1 (CX3CL1 Produkte)

Synonyme: ABCD3 ELISA Kit, CX3CL1 ELISA Kit, DKFZp459K117 ELISA Kit, Cx3c ELISA Kit, Scyd1 ELISA Kit, ABCD-3 ELISA Kit, C3Xkine ELISA Kit, CXC3 ELISA Kit, CXC3C ELISA Kit, NTN ELISA Kit, NTT ELISA Kit, SCYD1 ELISA Kit, fractalkine ELISA Kit, neurotactin ELISA Kit, AB030188 ELISA Kit, AI848747 ELISA Kit, CX3C ELISA Kit, Cxc3 ELISA Kit, D8Bwg0439e ELISA Kit, ATP binding cassette subfamily D member 3 ELISA Kit, C-X3-C motif chemokine ligand 1 ELISA Kit, chemokine (C-X3-C motif) ligand 1 ELISA Kit, ABCD3 ELISA Kit, CX3CL1 ELISA Kit, Cx3cl1 ELISA Kit, Abcd3 ELISA Kit

Gen-ID: 6376

NCBI Accession: NP_002987

UniProt: A0N0N7

Pathways: Synaptic Membrane