CYR61 ELISA Kit

Produktnummer ABIN6720366

Hersteller Produkt- Nr.: koa0700

Produktdetails CYR61 ELISA Kit

Target: CYR61 (Cysteine-Rich, Angiogenic Inducer, 61 (CYR61))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 31.2 pg/mL - 2000 pg/mL

Untere Nachweisgrenze: 31.2 pg/mL

Applikation: ELISA

Hersteller Produkt- Nr.: koa0700

Hersteller: Rockland

Verwendungszweck: Human CYR61 - CCN1 Sandwich ELISA Kit for Quantitative Detection

Marke: AccuSignal™

Proben: Breast Milk, Cell Culture Supernatant, Plasma (EDTA - heparin), Saliva, Serum, Urine

Analytische Methode: Quantitative

Spezifität:

Production: Natural and recombinant human CYR61. There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 10 pg/mL

Bestandteile:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Anwendungsinformationen

Applikationshinweise: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human CYR61 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA), saliva, urine or human milk to each empty well. It is recommended that each human CYR61 standard solution and each sample be measured in duplicate.

Kommentare:

Standard: Expression system for standard: CHO, Immunogen sequence: A22-D381

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu CYR61

Target: CYR61 (Cysteine-Rich, Angiogenic Inducer, 61 (CYR61))

Andere Bezeichnung: CYR61/CCN1 (CYR61 Produkte)

Hintergrund:

Synonyms: 3CH61, CCN 1, CCN family member 1, CCN1, CCN1, CYR 61, Cyr61, CYR61 protein, CYR61 protein precursor, CYR61 protein precursor, CYR61_HUMAN, Cysteine rich angiogenic inducer 61, Cysteine rich heparin binding protein 61, Cysteine-rich angiogenic inducer 61, GIG 1, GIG1, GIG1 protein, IBP-10, IGF-binding protein 10, Igfbp 10, IGFBP-10, IGFBP10, Insulin like growth factor binding protein 10, Insulin-like growth factor-binding protein 10, Protein CYR61, Protein GIG1

Background: Cysteine-rich angiogenic inducer 61(CYR61) or CCN family member 1(CCN1), is a matricellular protein that in humans is encoded by the CYR61 gene. This gene is mapped to 1p22.3. CYR61 is capable of regulating a broad range of cellular activities, including cell adhesion, migration, proliferation, differentiation, apoptosis, and senescence through interaction with cell surface integrin receptors and heparan sulfate proteoglycans. During embryonic development, CYR61 is critical for cardiac septal morphogenesis, blood vessel formation in placenta, and vascular integrity. In adulthood CYR61 plays important roles in inflammation and tissue repair, and is associated with diseases related to chronic inflammation, including rheumatoid arthritis, atherosclerosis, diabetes-related nephropathy and retinopathy, and many different forms of cancers.

Gen-ID: 3491

NCBI Accession: NP_001545

UniProt: O00622

Pathways: Positive Regulation of Endopeptidase Activity, Growth Factor Binding