EGF ELISA Kit

Produktnummer ABIN6720371

Produktdetails EGF ELISA Kit

Target: EGF (Epidermal Growth Factor (EGF))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 7.8 pg/mL - 500 pg/mL

Untere Nachweisgrenze: 7.8 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich ELISA for Quantitative Detection of Antigen

Proben: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Tissue Homogenate, Urine

Analytische Methode: Quantitative

Produktmerkmale:

Synonyms: AI790464, Beta urogastrone, Epidermal growth factor, HOMG4, OTTHUMP00000219721, OTTHUMP00000219722, Pro epidermal growth factor, Pro epidermal growth factor precursor (EGF), URG, Urogastrone

Background: Epidermal growth factor(EGF) is a growth factor that plays an important role in the regulation of cell growth, proliferation and differentiation by binding to its receptor EGFR. EGF locus is mapped to 4q21-4qter. Human EGF is a 6045-Da protein with 53 amino acid residues and three intramolecular disulfide bones. EGF results in cellular proliferation, differentiation, and survival while plays a physiological role in the maintenance of oro-esophageal and gastric tissue integrity.

Gene Name: EGF

Production: Natural and recombinant rat EGF. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: E.coli, Immunogen sequence: N974-R1026

Anwendungsinformationen

Applikationshinweise:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL, 7.8pg/mL rat EGF standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum, plasma(heparin, EDTA), tissue homogenates or urine to each empty well. It is recommended that each rat EGF standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 7.8pg/mL-500pg/mL

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Details zu EGF

Target: EGF (Epidermal Growth Factor (EGF))

Andere Bezeichnung: EGF (EGF Produkte)

Synonyme: HOMG4 ELISA Kit, URG ELISA Kit, AI790464 ELISA Kit, CEGF ELISA Kit, epidermal growth factor ELISA Kit, pro-epidermal growth factor ELISA Kit, EGF ELISA Kit, egf ELISA Kit, CpipJ_CPIJ020278 ELISA Kit, Egf ELISA Kit

Gen-ID: 25313

NCBI Accession: NP_036974

UniProt: P07522

Pathways: NF-kappaB Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation