TEK ELISA Kit

Produktnummer ABIN6720391

Produktdetails TEK ELISA Kit

Target: TEK (TEK Tyrosine Kinase, Endothelial (TEK))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 125 pg/mL - 8000 pg/mL

Untere Nachweisgrenze: 125 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich ELISA for Quantitative Detection of Antigen

Proben: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Tissue Homogenate

Analytische Methode: Quantitative

Produktmerkmale:

Synonyms: Angiopoietin-1 receptor, CD202b, CD202b antigen, EC 2.7.10.1, hTIE2, p140 TEK, soluble TIE2, variant 1, soluble TIE2 variant 2, Tek, TEK tyrosine kinase, endothelial, TIE 2, TIE2_HUMAN, Tunica interna endothelial cell kinase, Tyrosine-protein kinase receptor TEK, Tyrosine-protein kinase receptor TIE 2, Tyrosine-protein kinase receptor TIE-2, venous malformations, multiple cutaneous and mucosal, VMCM, VMCM1

Background: Angiopoietin-1 receptor, also called TEK or TIE2 is a protein that in humans is encoded by the TEK gene. This gene is mapped to 9p21.2. The TEK receptor tyrosine kinase is expressed almost exclusively in endothelial cells in mice, rats, and humans. This receptor possesses a unique extracellular domain containing 2 immunoglobulin-like loops separated by 3 epidermal growth factor-like repeats that are connected to 3 fibronectin type III-like repeats. The ligand for the receptor is angiopoietin-1. Defects in TEK are associated with inherited venous malformations, the TEK signaling pathway appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis. TEK is closely related to the TIE receptor tyrosine kinase.

Gene Name: TEK

Production: Natural and recombinant mouse TEK. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: A23-K744

Anwendungsinformationen

Applikationshinweise:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 8000pg/mL, 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL mouse TEK standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum, tissue homogenates or plasma (heparin, EDTA) to each empty well. It is recommended that each mouse TEK standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 125pg/mL-8000pg/mL

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Details zu TEK

Target: TEK (TEK Tyrosine Kinase, Endothelial (TEK))

Andere Bezeichnung: TEK - TIE2 (TEK Produkte)

Synonyme: TEK ELISA Kit, DKFZp469E064 ELISA Kit, AA517024 ELISA Kit, Cd202b ELISA Kit, Hyk ELISA Kit, Tie2 ELISA Kit, tie-2 ELISA Kit, CD202B ELISA Kit, TIE-2 ELISA Kit, TIE2 ELISA Kit, VMCM ELISA Kit, VMCM1 ELISA Kit, Tie-2 ELISA Kit, tie2 ELISA Kit, TEK receptor tyrosine kinase ELISA Kit, angiopoietin-1 receptor ELISA Kit, TEK tyrosine kinase, endothelial ELISA Kit, TEK ELISA Kit, LOC100408334 ELISA Kit, tek ELISA Kit, Tek ELISA Kit

Gen-ID: 21687

NCBI Accession: NP_001277478

UniProt: Q02858

Pathways: RTK Signalweg, Growth Factor Binding