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FURIN ELISA Kit

Rockland koa0553 FURIN Reaktivität: Human Colorimetric Sandwich ELISA 312 pg/mL - 20000 pg/mL Cell Culture Lysate, Cell Culture Supernatant, Tissue Homogenate
Rockland
Produktnummer ABIN6720393
Hersteller Produkt- Nr.: koa0553
  • Target Alle FURIN ELISA Kits anzeigen
    FURIN (Furin (Paired Basic Amino Acid Cleaving Enzyme) (FURIN))
    Reaktivität
    • 8
    • 2
    • 2
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    312 pg/mL - 20000 pg/mL
    Untere Nachweisgrenze
    312 pg/mL
    Applikation
    ELISA
    Hersteller Produkt- Nr.
    koa0553
    Hersteller
    Rockland
    Verwendungszweck
    Human Furin Sandwich ELISA Kit for Quantitative Detection
    Marke
    AccuSignal™
    Proben
    Cell Culture Lysate, Cell Culture Supernatant, Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität

    Production: Natural and recombinant human Furin. There is no detectable cross-reactivity with other relevant proteins.

    Sensitivität
    < 10 pg/mL
    Bestandteile
    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
    Top Product
    Discover our top product FURIN ELISA Kit
  • Applikationshinweise
    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 20000pg/mL, 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human Furin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, tissue homogenates or cell lysates to each empty well. It is recommended that each human Furin standard solution and each sample be measured in duplicate.
    Kommentare

    Standard: Expression system for standard: NSO, Immunogen sequence: D108-E715

    Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
    Haltbarkeit
    12 months
  • Target Alle FURIN ELISA Kits anzeigen
    FURIN (Furin (Paired Basic Amino Acid Cleaving Enzyme) (FURIN))
    Andere Bezeichnung
    Furin (FURIN Produkte)
    Hintergrund

    Synonyms: Dibasic processing enzyme, Dibasic-processing enzyme, FES upstream region, FUR, FURIN, Furin membrane associated receptor protein, FURIN_HUMAN, PACE, Paired basic amino acid residue cleaving enzyme, Paired basic amino acid residue-cleaving enzyme, PCSK3, Proprotein convertase subtilisin/kexin type 3, SPC1

    Background: Furin (Fur), also known as PACE, is a protein that in humans is encoded by the FURIN gene. The protein encoded by this gene is an enzyme which belongs to the subtilisin-like proprotein convertase family. The FUR gene is located approximately 1 kb upstream of the FES gene on chromosome 15q25-q26. This gene is thought to play a role in tumor progression. Furin is one of the proteases responsible for the proteolytic cleavage of HIV envelope polyprotein precursor gp160 to gp120 and gp41 prior to viral assembly. Furin is enriched in the Golgi apparatus, where it functions to cleave other proteins into their mature/active forms. Expression of furin in T-cells is required for maintenance of peripheral immune tolerance.

    Gen-ID
    5045
    NCBI Accession
    NP_001276752
    UniProt
    P09958
    Pathways
    Notch Signalweg, Neurotrophin Signalübertragung
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