FURIN ELISA Kit

Produktnummer ABIN6720393

Produktdetails FURIN ELISA Kit

Target: FURIN (Furin (Paired Basic Amino Acid Cleaving Enzyme) (FURIN))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 312 pg/mL - 20000 pg/mL

Untere Nachweisgrenze: 312 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich ELISA for Quantitative Detection of Antigen

Proben: Cell Culture Lysate, Cell Culture Supernatant, Tissue Homogenate

Analytische Methode: Quantitative

Produktmerkmale:

Synonyms: Dibasic processing enzyme, Dibasic-processing enzyme, FES upstream region, FUR, FURIN, Furin membrane associated receptor protein, FURIN_HUMAN, PACE, Paired basic amino acid residue cleaving enzyme, Paired basic amino acid residue-cleaving enzyme, PCSK3, Proprotein convertase subtilisin/kexin type 3, SPC1

Background: Furin(Fur), also known as PACE, is a protein that in humans is encoded by the FURIN gene. The protein encoded by this gene is an enzyme which belongs to the subtilisin-like proprotein convertase family. The FUR gene is located approximately 1 kb upstream of the FES gene on chromosome 15q25-q26. This gene is thought to play a role in tumor progression. Furin is one of the proteases responsible for the proteolytic cleavage of HIV envelope polyprotein precursor gp160 to gp120 and gp41 prior to viral assembly. Furin is enriched in the Golgi apparatus, where it functions to cleave other proteins into their mature/active forms. Expression of furin in T-cells is required for maintenance of peripheral immune tolerance

Gene Name: FURIN

Production: Natural and recombinant human Furin. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: D108-E715

Anwendungsinformationen

Applikationshinweise:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 20000pg/mL, 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human Furin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, tissue homogenates or cell lysates to each empty well. It is recommended that each human Furin standard solution and each sample be measured in duplicate.

ELISA Dilution: 312pg/mL-20000pg/mL

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Details zu FURIN

Target: FURIN (Furin (Paired Basic Amino Acid Cleaving Enzyme) (FURIN))

Andere Bezeichnung: Furin (FURIN Produkte)

Synonyme: FURIN ELISA Kit, furin ELISA Kit, si:dkey-281a24.5 ELISA Kit, FUR ELISA Kit, LOC100220259 ELISA Kit, PACE ELISA Kit, spc1 ELISA Kit, xfurin ELISA Kit, MGC82710 ELISA Kit, PCSK3 ELISA Kit, SPC1 ELISA Kit, 9130404I01Rik ELISA Kit, Fur ELISA Kit, Pcsk3 ELISA Kit, Pace ELISA Kit, furin, paired basic amino acid cleaving enzyme ELISA Kit, furin (paired basic amino acid cleaving enzyme) a ELISA Kit, furin (paired basic amino acid cleaving enzyme) b ELISA Kit, furin, paired basic amino acid cleaving enzyme L homeolog ELISA Kit, furin (paired basic amino acid cleaving enzyme) ELISA Kit, FURIN ELISA Kit, furina ELISA Kit, furinb ELISA Kit, furin ELISA Kit, furin.L ELISA Kit, Furin ELISA Kit

Gen-ID: 5045

NCBI Accession: NP_001276752

UniProt: P09958

Pathways: Notch Signalweg, Neurotrophin Signalübertragung