FURIN ELISA Kit
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- Target Alle FURIN ELISA Kits anzeigen
- FURIN (Furin (Paired Basic Amino Acid Cleaving Enzyme) (FURIN))
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Reaktivität
- Human
- Nachweismethode
- Colorimetric
- Methodentyp
- Sandwich ELISA
- Detektionsbereich
- 312 pg/mL - 20000 pg/mL
- Untere Nachweisgrenze
- 312 pg/mL
- Applikation
- ELISA
- Hersteller Produkt- Nr.
- koa0553
- Hersteller
- Rockland
- Verwendungszweck
- Human Furin Sandwich ELISA Kit for Quantitative Detection
- Marke
- AccuSignal™
- Proben
- Cell Culture Lysate, Cell Culture Supernatant, Tissue Homogenate
- Analytische Methode
- Quantitative
- Spezifität
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Production: Natural and recombinant human Furin. There is no detectable cross-reactivity with other relevant proteins.
- Sensitivität
- < 10 pg/mL
- Bestandteile
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
- Top Product
- Discover our top product FURIN ELISA Kit
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- Applikationshinweise
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 20000pg/mL, 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human Furin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, tissue homogenates or cell lysates to each empty well. It is recommended that each human Furin standard solution and each sample be measured in duplicate.
- Kommentare
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Standard: Expression system for standard: NSO, Immunogen sequence: D108-E715
- Probenmenge
- 100 μL
- Plattentyp
- Pre-coated
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
- Haltbarkeit
- 12 months
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- Target Alle FURIN ELISA Kits anzeigen
- FURIN (Furin (Paired Basic Amino Acid Cleaving Enzyme) (FURIN))
- Andere Bezeichnung
- Furin (FURIN Produkte)
- Hintergrund
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Synonyms: Dibasic processing enzyme, Dibasic-processing enzyme, FES upstream region, FUR, FURIN, Furin membrane associated receptor protein, FURIN_HUMAN, PACE, Paired basic amino acid residue cleaving enzyme, Paired basic amino acid residue-cleaving enzyme, PCSK3, Proprotein convertase subtilisin/kexin type 3, SPC1
Background: Furin (Fur), also known as PACE, is a protein that in humans is encoded by the FURIN gene. The protein encoded by this gene is an enzyme which belongs to the subtilisin-like proprotein convertase family. The FUR gene is located approximately 1 kb upstream of the FES gene on chromosome 15q25-q26. This gene is thought to play a role in tumor progression. Furin is one of the proteases responsible for the proteolytic cleavage of HIV envelope polyprotein precursor gp160 to gp120 and gp41 prior to viral assembly. Furin is enriched in the Golgi apparatus, where it functions to cleave other proteins into their mature/active forms. Expression of furin in T-cells is required for maintenance of peripheral immune tolerance.
- Gen-ID
- 5045
- NCBI Accession
- NP_001276752
- UniProt
- P09958
- Pathways
- Notch Signalweg, Neurotrophin Signalübertragung
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