Adenylate Kinase 2 ELISA Kit

Produktnummer ABIN6730062

Produktdetails Adenylate Kinase 2 ELISA Kit

Target: Adenylate Kinase 2 (AK2)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human AK2 ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair recognizes Human AK2.

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu Adenylate Kinase 2

Target: Adenylate Kinase 2 (AK2)

Andere Bezeichnung: AK2 (AK2 Produkte)

Synonyme: ADK-2 ELISA Kit, BcDNA:SD09634 ELISA Kit, CG3140 ELISA Kit, Dak2 ELISA Kit, Dmel\\CG3140 ELISA Kit, anon-Dak2 ELISA Kit, adk2 ELISA Kit, NV10896 ELISA Kit, wu:fb34f05 ELISA Kit, wu:fj80e03 ELISA Kit, ADK2 ELISA Kit, AK 2 ELISA Kit, Ak-2 ELISA Kit, D4Ertd220e ELISA Kit, MXI22.8 ELISA Kit, MXI22_8 ELISA Kit, Adenylate kinase 2 ELISA Kit, adenylate kinase 2 ELISA Kit, adenylate kinase ELISA Kit, adenylate kinase 2 S homeolog ELISA Kit, Adenylate kinase family protein ELISA Kit, Adk2 ELISA Kit, AK2 ELISA Kit, ak2 ELISA Kit, LOC100123971 ELISA Kit, ak2.S ELISA Kit, Ak2 ELISA Kit, AT5G50370 ELISA Kit

UniProt: P54819

Pathways: Nucleotide Phosphorylation, Ribonucleoside Biosynthetic Process