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TGFBR1 ELISA Kit
TGFBR1
Reaktivität: Maus
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Produktnummer ABIN6730211
Produktdetails TGFBR1 ELISA Kit
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Target
Alle TGFBR1 ELISA Kits anzeigen
TGFBR1
(Transforming Growth Factor, beta Receptor 1 (TGFBR1))
Reaktivität
Alle Reaktivitäten für TGFBR1 ELISA Kits
Maus
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Applikation
ELISA
Verwendungszweck
Mouse TGF-beta RI/ALK-5 ELISA Kit.
Proben
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Analytische Methode
Quantitative
Spezifität
This ELISA antibody pair recognizes Mouse TGF-beta RI.
Produktmerkmale
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 µl to 1 µl volumes Adjustable 1-25 µl pipettes for reagent preparation 100 µl and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Discover our top product TGFBR1 ELISA Kit
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Anwendungsinformationen
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Applikationshinweise
Optimal working dilution should be determined by the investigator.
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 100 µl of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4°C. Add 100 µl of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 µl of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 µl of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 µl of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Informationen zur Lagerung
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit
6 months
Details zu TGFBR1
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Target
Alle TGFBR1 ELISA Kits anzeigen
TGFBR1
(Transforming Growth Factor, beta Receptor 1 (TGFBR1))
Andere Bezeichnung
TGF-beta RI (ALK-5 (TGFBR1 Produkte )
Synonyme
AAT5 ELISA Kit, ACVRLK4 ELISA Kit, ALK-5 ELISA Kit, ALK5 ELISA Kit, LDS1A ELISA Kit, LDS2A ELISA Kit, MSSE ELISA Kit, SKR4 ELISA Kit, TGFR-1 ELISA Kit, aat5 ELISA Kit, acvrlk4 ELISA Kit, alk-5 ELISA Kit, alk5 ELISA Kit, lds1a ELISA Kit, lds2a ELISA Kit, skr4 ELISA Kit, tgfr-1 ELISA Kit, TGFBR1 ELISA Kit, x-trr1 ELISA Kit, AU017191 ELISA Kit, Alk-5 ELISA Kit, TbetaR-I ELISA Kit, TbetaRI ELISA Kit, Alk5 ELISA Kit, Skr4 ELISA Kit, Tgfr-1 ELISA Kit, tbetaR-I ELISA Kit, tgfbr1 ELISA Kit, zgc:123263 ELISA Kit, transforming growth factor beta receptor 1 ELISA Kit, transforming growth factor beta receptor I ELISA Kit, transforming growth factor beta receptor I S homeolog ELISA Kit, transforming growth factor, beta receptor I ELISA Kit, transforming growth factor, beta receptor 1 ELISA Kit, transforming growth factor, beta receptor 1 a ELISA Kit, TGFBR1 ELISA Kit, tgfbr1 ELISA Kit, tgfbr1.S ELISA Kit, Tgfbr1 ELISA Kit, tgfbr1a ELISA Kit
UniProt
Q64729
Pathways
Growth Factor Binding
Zuletzt angesehen
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