Netrin 1 ELISA Kit
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- Target Alle Netrin 1 (NTN1) ELISA Kits anzeigen
- Netrin 1 (NTN1)
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Reaktivität
- Maus
- Nachweismethode
- Colorimetric
- Methodentyp
- Sandwich ELISA
- Applikation
- ELISA
- Verwendungszweck
- Mouse Netrin-1 ELISA Kit.
- Proben
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytische Methode
- Quantitative
- Spezifität
- This ELISA antibody pair recognizes Mouse Netrin-1.
- Produktmerkmale
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Bestandteile
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Benötigtes Material
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product NTN1 ELISA Kit
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- Applikationshinweise
- Optimal working dilution should be determined by the investigator.
- Protokoll
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Informationen zur Lagerung
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- Haltbarkeit
- 6 months
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- Target Alle Netrin 1 (NTN1) ELISA Kits anzeigen
- Netrin 1 (NTN1)
- Andere Bezeichnung
- Netrin-1 (NTN1 Produkte)
- Synonyme
- ntn1-A ELISA Kit, Netrin-1 ELISA Kit, NTN1 ELISA Kit, unc-6 ELISA Kit, MGC147073 ELISA Kit, NTN1L ELISA Kit, AI561871 ELISA Kit, BC019633 ELISA Kit, netrin-1 ELISA Kit, netrin 1 L homeolog ELISA Kit, netrin 1 ELISA Kit, ntn1.L ELISA Kit, NTN1 ELISA Kit, ntn1 ELISA Kit, Ntn1 ELISA Kit
- UniProt
- O09118
- Pathways
- Regulation of Cell Size
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