RAB6A ELISA Kit

Produktnummer ABIN6730273

Produktdetails RAB6A ELISA Kit

Target: RAB6A (RAB6A, Member RAS Oncogene Family (RAB6A))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human RAB6A ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair recognizes Human RAB6A.

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu RAB6A

Target: RAB6A (RAB6A, Member RAS Oncogene Family (RAB6A))

Andere Bezeichnung: RAB6A (RAB6A Produkte)

Synonyme: fb43c01 ELISA Kit, wu:fb43c01 ELISA Kit, zgc:55788 ELISA Kit, rab6 ELISA Kit, MGC53077 ELISA Kit, rab6b ELISA Kit, rab6a' ELISA Kit, MGC76176 ELISA Kit, LOC692966 ELISA Kit, RAB6 ELISA Kit, 2610028L11Rik ELISA Kit, AA419671 ELISA Kit, Rab6 ELISA Kit, RCO4-3 ELISA Kit, Rab6b ELISA Kit, RAB6A, member RAS oncogene family ELISA Kit, RAB6A, member RAS oncogene family S homeolog ELISA Kit, ras small monomeric GTPase Rab6 ELISA Kit, ras-related protein Rab-6A ELISA Kit, rab6a ELISA Kit, rab6a.S ELISA Kit, LOC692966 ELISA Kit, RAB6A ELISA Kit, Rab6a ELISA Kit

UniProt: P20340