Sortilin 1 ELISA Kit

Produktnummer ABIN6730289

Produktdetails Sortilin 1 ELISA Kit

Target: Sortilin 1 (SORT1)

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Mouse Sortilin ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair recognizes Mouse Sortilin.

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu Sortilin 1

Target: Sortilin 1 (SORT1)

Andere Bezeichnung: Sortilin (SORT1 Produkte)

Synonyme: SORT1 ELISA Kit, 19.m02079 ELISA Kit, gp95 ELISA Kit, ldlcq6 ELISA Kit, sortilin ELISA Kit, Gp95 ELISA Kit, LDLCQ6 ELISA Kit, NT3 ELISA Kit, 2900053A11Rik ELISA Kit, AI852375 ELISA Kit, Ntr3 ELISA Kit, Ntsr3 ELISA Kit, Nt3 ELISA Kit, Nts3 ELISA Kit, sort1 ELISA Kit, zgc:85953 ELISA Kit, sortilin 1 ELISA Kit, sortilin ELISA Kit, sortilin 1 S homeolog ELISA Kit, sortilin 1a ELISA Kit, SORT1 ELISA Kit, TP01_0016 ELISA Kit, BBOV_I002730 ELISA Kit, sort1.S ELISA Kit, Sort1 ELISA Kit, sort1a ELISA Kit

UniProt: Q8BI61

Pathways: Neurotrophin Signalübertragung