Calcineurin B ELISA Kit

Produktnummer ABIN6730337

Produktdetails Calcineurin B ELISA Kit

Target: Calcineurin B (CAN)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human Calcineurin B ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair recognizes Human Calcineurin B.

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu Calcineurin B

Target: Calcineurin B (CAN)

Andere Bezeichnung: Calcineurin B (CAN Produkte)

Synonyme: PPP3RL ELISA Kit, BcDNA:RH11383 ELISA Kit, CG4209 ELISA Kit, CNB ELISA Kit, Can ELISA Kit, CnB ELISA Kit, Dmel\\CG4209 ELISA Kit, canB-4F ELISA Kit, dCnB1 ELISA Kit, Cnb ELISA Kit, Cblp ELISA Kit, CaNB2 ELISA Kit, protein phosphatase 3 regulatory subunit B, beta ELISA Kit, Calcineurin B ELISA Kit, calcineurin B ELISA Kit, protein phosphatase 3, regulatory subunit B, beta ELISA Kit, protein phosphatase 3 regulatory subunit B, alpha ELISA Kit, protein phosphatase 3, regulatory subunit B, alpha isoform (calcineurin B, type II) ELISA Kit, PPP3R2 ELISA Kit, CanB ELISA Kit, Cnb ELISA Kit, Ppp3r2 ELISA Kit, PPP3R1 ELISA Kit

UniProt: Q96LZ3

Pathways: Cellular Glucan Metabolic Process, VEGF Signaling