KLKB1 ELISA Kit

Produktnummer ABIN6730355

Produktdetails KLKB1 ELISA Kit

Target: KLKB1 (Kallikrein B, Plasma (Fletcher Factor) 1 (KLKB1))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Mouse KLKB1 ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair recognizes Mouse KLKB1.

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu KLKB1

Target: KLKB1 (Kallikrein B, Plasma (Fletcher Factor) 1 (KLKB1))

Andere Bezeichnung: KLKB1 (KLKB1 Produkte)

Synonyme: KLK3 ELISA Kit, PPK ELISA Kit, APS ELISA Kit, Kal-3 ELISA Kit, Kal3 ELISA Kit, Klk3 ELISA Kit, PSA ELISA Kit, KALP15 ELISA Kit, Kalp1 ELISA Kit, KLKB1 ELISA Kit, DKFZp459J2015 ELISA Kit, klkb1 ELISA Kit, kallikrein B1 ELISA Kit, kallikrein B, plasma 1 ELISA Kit, plasma kallikrein ELISA Kit, Plasma kallikrein ELISA Kit, kallikrein B, plasma (Fletcher factor) 1 ELISA Kit, coagulation factor XI ELISA Kit, kallikrein B1 L homeolog ELISA Kit, KLKB1 ELISA Kit, Klkb1 ELISA Kit, CpipJ_CPIJ004042 ELISA Kit, CpipJ_CPIJ014955 ELISA Kit, CpipJ_CPIJ020108 ELISA Kit, VAA_02266 ELISA Kit, LOC100549617 ELISA Kit, klkb1 ELISA Kit, klkb1.L ELISA Kit

UniProt: P26262

Pathways: Komplementsystem, Brown Fat Cell Differentiation