PLA2G1B ELISA Kit

Produktnummer ABIN6730357

Produktdetails PLA2G1B ELISA Kit

Target: PLA2G1B (Phospholipase A2, Group IB (PLA2G1B))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Mouse PLA2G1B ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair recognizes Mouse PLA2G1B.

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu PLA2G1B

Target: PLA2G1B (Phospholipase A2, Group IB (PLA2G1B))

Andere Bezeichnung: PLA2G1B (PLA2G1B Produkte)

Synonyme: C133 ELISA Kit, CG4346 ELISA Kit, Dmel\\CG42237 ELISA Kit, Dmel_CG4346 ELISA Kit, PLA2 ELISA Kit, GB13351 ELISA Kit, bvPLA2 ELISA Kit, PLA2A ELISA Kit, PPLA2 ELISA Kit, sPLA(2)-IB ELISA Kit, Pla2a ELISA Kit, sPLA2IB ELISA Kit, PLA2G1B ELISA Kit, CG42237 gene product from transcript CG42237-RB ELISA Kit, phospholipase A2 ELISA Kit, phospholipase A2 group IB ELISA Kit, phospholipase A2, group IB, pancreas ELISA Kit, phospholipase A2 group IVA ELISA Kit, CG42237 ELISA Kit, PLA2 ELISA Kit, LOC100631065 ELISA Kit, Pla2 ELISA Kit, PLA2G1B ELISA Kit, Pla2g1b ELISA Kit, PLA2G4A ELISA Kit

UniProt: Q9Z0Y2

Pathways: Inositol Metabolic Process, VEGF Signaling