CRABP1 ELISA Kit

Produktnummer ABIN6730371

Produktdetails CRABP1 ELISA Kit

Target: CRABP1 (Cellular Retinoic Acid Binding Protein 1 (CRABP1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human CRABP1 ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair recognizes Human CRABP1.

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu CRABP1

Target: CRABP1 (Cellular Retinoic Acid Binding Protein 1 (CRABP1))

Andere Bezeichnung: CRABP1 (CRABP1 Produkte)

Synonyme: CRABP ELISA Kit, CRABP-I ELISA Kit, CRABPI ELISA Kit, RBP5 ELISA Kit, CRABP1 ELISA Kit, cCRABP-I ELISA Kit, AI326249 ELISA Kit, Crabp-1 ELISA Kit, CrabpI ELISA Kit, Rbp-5 ELISA Kit, crabp1l ELISA Kit, crabpib ELISA Kit, crabp1 ELISA Kit, zgc:110497 ELISA Kit, cellular retinoic acid binding protein 1 ELISA Kit, cellular retinoic acid binding protein 1 S homeolog ELISA Kit, cellular retinoic acid binding protein I ELISA Kit, cellular retinoic acid binding protein 1b ELISA Kit, cellular retinoic acid binding protein 1a ELISA Kit, CRABP1 ELISA Kit, Crabp1 ELISA Kit, crabp1.S ELISA Kit, crabp1 ELISA Kit, crabp1b ELISA Kit, crabp1a ELISA Kit

UniProt: P29762