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CAMK4 ELISA Kit
CAMK4
Reaktivität: Maus
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Produktnummer ABIN6730427
Produktdetails CAMK4 ELISA Kit
(ausblenden)
Target
Alle CAMK4 ELISA Kits anzeigen
CAMK4
(Calcium/calmodulin-Dependent Protein Kinase IV (CAMK4))
Reaktivität
Alle Reaktivitäten für CAMK4 ELISA Kits
Maus
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Applikation
ELISA
Verwendungszweck
Mouse CAMK4 ELISA Kit.
Proben
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Analytische Methode
Quantitative
Spezifität
This ELISA antibody pair recognizes Mouse CAMK4.
Produktmerkmale
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 µl to 1 µl volumes Adjustable 1-25 µl pipettes for reagent preparation 100 µl and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Anwendungsinformationen
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Applikationshinweise
Optimal working dilution should be determined by the investigator.
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 100 µl of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4°C. Add 100 µl of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 µl of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 µl of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 µl of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Informationen zur Lagerung
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit
6 months
Details zu CAMK4
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Target
Alle CAMK4 ELISA Kits anzeigen
CAMK4
(Calcium/calmodulin-Dependent Protein Kinase IV (CAMK4))
Andere Bezeichnung
CAMK4 (CAMK4 Produkte )
Synonyme
CaMK IV ELISA Kit, CaMK-GR ELISA Kit, IV ELISA Kit, caMK ELISA Kit, CAMK4 ELISA Kit, A430110E23Rik ELISA Kit, AI666733 ELISA Kit, CaMKIV ELISA Kit, CaMKIV/Gr ELISA Kit, D18Bwg0362e ELISA Kit, Ccdpk ELISA Kit, RATCCDPK ELISA Kit, camk4-A ELISA Kit, wu:fj42a03 ELISA Kit, zgc:110275 ELISA Kit, calcium/calmodulin dependent protein kinase IV ELISA Kit, calcium/calmodulin-dependent protein kinase IV ELISA Kit, calcium/calmodulin-dependent protein kinase IV L homeolog ELISA Kit, CAMK4 ELISA Kit, Camk4 ELISA Kit, camk4.L ELISA Kit, camk4 ELISA Kit
UniProt
P08414
Pathways
EGFR Signaling Pathway , Neurotrophin Signalübertragung , Production of Molecular Mediator of Immune Response , G-protein mediated Events , Interaction of EGFR with phospholipase C-gamma
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