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PDGFRA ELISA Kit
PDGFRA
Reaktivität: Maus
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Produktnummer ABIN6730429
Produktdetails PDGFRA ELISA Kit
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Target
Alle PDGFRA ELISA Kits anzeigen
PDGFRA
(Platelet Derived Growth Factor Receptor alpha (PDGFRA))
Reaktivität
Alle Reaktivitäten für PDGFRA ELISA Kits
Maus
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Applikation
ELISA
Verwendungszweck
Mouse PDGF R alpha ELISA Kit.
Proben
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Analytische Methode
Quantitative
Spezifität
This ELISA antibody pair recognizes Mouse PDGF R alpha.
Produktmerkmale
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 µl to 1 µl volumes Adjustable 1-25 µl pipettes for reagent preparation 100 µl and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Anwendungsinformationen
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Applikationshinweise
Optimal working dilution should be determined by the investigator.
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 100 µl of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4°C. Add 100 µl of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 µl of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 µl of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 µl of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Informationen zur Lagerung
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit
6 months
Details zu PDGFRA
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Target
Alle PDGFRA ELISA Kits anzeigen
PDGFRA
(Platelet Derived Growth Factor Receptor alpha (PDGFRA))
Andere Bezeichnung
PDGF R alpha (PDGFRA Produkte )
Synonyme
AI115593 ELISA Kit, CD140a ELISA Kit, Pdgfr-2 ELISA Kit, APDGFR ELISA Kit, PDGFACE ELISA Kit, CD140A ELISA Kit, PDGFR-2 ELISA Kit, PDGFR2 ELISA Kit, RHEPDGFRA ELISA Kit, PDGFR ELISA Kit, PDGFR-A ELISA Kit, platelet derived growth factor receptor alpha ELISA Kit, platelet derived growth factor receptor, alpha polypeptide ELISA Kit, platelet-derived growth factor receptor, alpha polypeptide L homeolog ELISA Kit, platelet-derived growth factor receptor, alpha polypeptide ELISA Kit, PDGFRA ELISA Kit, Pdgfra ELISA Kit, pdgfra.L ELISA Kit
UniProt
P26618
Pathways
RTK Signalweg , Fc-epsilon Rezeptor Signalübertragung , EGFR Signaling Pathway , Neurotrophin Signalübertragung , Platelet-derived growth Factor Receptor Signaling
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