ICOSLG ELISA Kit

Produktnummer ABIN6730439

Produktdetails ICOSLG ELISA Kit

Target: ICOSLG (Inducible T-Cell Co-Stimulator Ligand (ICOSLG))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Mouse B7-H2/ICOS Ligand ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair recognizes Mouse B7-H2.

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu ICOSLG

Target: ICOSLG (Inducible T-Cell Co-Stimulator Ligand (ICOSLG))

Andere Bezeichnung: B7-H2 (ICOS Ligand (ICOSLG Produkte)

Synonyme: ICOSLG ELISA Kit, AU044799 ELISA Kit, B7-H2 ELISA Kit, B7RP-1 ELISA Kit, B7h ELISA Kit, BG071784 ELISA Kit, GI50 ELISA Kit, GL50 ELISA Kit, GL50-B ELISA Kit, ICOS-L ELISA Kit, Icoslg ELISA Kit, LICOS ELISA Kit, Ly115l ELISA Kit, mKIAA0653 ELISA Kit, B7H2 ELISA Kit, B7RP1 ELISA Kit, CD275 ELISA Kit, ICOSL ELISA Kit, Icosl ELISA Kit, RGD1562791 ELISA Kit, inducible T-cell costimulator ligand ELISA Kit, icos ligand ELISA Kit, inducible T-cell co-stimulator ligand ELISA Kit, ICOSLG ELISA Kit, Icosl ELISA Kit, Icoslg ELISA Kit

UniProt: Q9JHJ8

Pathways: Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation