Galectin 9 ELISA Kit

Produktnummer ABIN6730490

Produktdetails Galectin 9 ELISA Kit

Target: Galectin 9 (LGALS9) (Lectin, Galactoside-Binding, Soluble, 9 (LGALS9))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Mouse Galectin-9 ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair recognizes Mouse Galectin-9.

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu Galectin 9

Target: Galectin 9 (LGALS9) (Lectin, Galactoside-Binding, Soluble, 9 (LGALS9))

Andere Bezeichnung: Galectin-9 (LGALS9 Produkte)

Synonyme: huat ELISA Kit, lgals9a ELISA Kit, ecalectin ELISA Kit, galectin-9 ELISA Kit, Lgals9 ELISA Kit, wu:fd20c09 ELISA Kit, zgc:111833 ELISA Kit, LGALS9 ELISA Kit, AA407335 ELISA Kit, AI194909 ELISA Kit, AI265545 ELISA Kit, LGALS35 ELISA Kit, Lgals5 ELISA Kit, gal-9 ELISA Kit, HUAT ELISA Kit, LGALS9A ELISA Kit, UAT ELISA Kit, UATP.I ELISA Kit, galectin 9 L homeolog ELISA Kit, galectin 9 ELISA Kit, lectin, galactoside-binding, soluble, 9 (galectin 9)-like 3 ELISA Kit, lectin, galactose binding, soluble 9 ELISA Kit, lgals9.L ELISA Kit, lgals9 ELISA Kit, lgals9l3 ELISA Kit, LGALS9 ELISA Kit, Lgals9 ELISA Kit

UniProt: O08573