MEK2 ELISA Kit

Produktnummer ABIN6730501

Produktdetails MEK2 ELISA Kit

Target: MEK2 (MAP2K2) (Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human MEK2/MAP2K2 ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair recognizes Human MEK2.

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu MEK2

Target: MEK2 (MAP2K2) (Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2))

Andere Bezeichnung: MEK2 (MAP2K2 (MAP2K2 Produkte)

Synonyme: CFC4 ELISA Kit, MAPKK2 ELISA Kit, MEK2 ELISA Kit, MKK2 ELISA Kit, PRKMK2 ELISA Kit, AA589381 ELISA Kit, MK2 ELISA Kit, Prkmk2 ELISA Kit, ATMKK2 ELISA Kit, F27B13.50 ELISA Kit, F27B13_50 ELISA Kit, MAP KINASE KINASE 1 ELISA Kit, MAP kinase kinase 2 ELISA Kit, MK1 ELISA Kit, map2k2 ELISA Kit, wu:fa56c06 ELISA Kit, zgc:123171 ELISA Kit, zgc:172250 ELISA Kit, mitogen-activated protein kinase kinase 2 ELISA Kit, mitogen activated protein kinase kinase 2 ELISA Kit, MAP kinase kinase 2 ELISA Kit, mitogen-activated protein kinase kinase 2a ELISA Kit, mitogen-activated protein kinase kinase 2b ELISA Kit, MAP2K2 ELISA Kit, Map2k2 ELISA Kit, MKK2 ELISA Kit, map2k2a ELISA Kit, map2k2b ELISA Kit

UniProt: P36507

Pathways: MAPK Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, Activation of Innate immune Response, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling