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CD13 ELISA Kit

ANPEP Reaktivität: Maus Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Produktnummer ABIN6730573
  • Target Alle CD13 (ANPEP) ELISA Kits anzeigen
    CD13 (ANPEP) (Alanyl (Membrane) Aminopeptidase (ANPEP))
    Reaktivität
    • 5
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    Maus
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Mouse Aminopeptidase N/ANPEP ELISA Kit.
    Proben
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytische Methode
    Quantitative
    Spezifität
    This ELISA antibody pair recognizes Mouse Aminopeptidase N.
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Featured
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  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Informationen zur Lagerung
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Haltbarkeit
    6 months
  • Target Alle CD13 (ANPEP) ELISA Kits anzeigen
    CD13 (ANPEP) (Alanyl (Membrane) Aminopeptidase (ANPEP))
    Andere Bezeichnung
    Aminopeptidase N (ANPEP (ANPEP Produkte)
    Synonyme
    APN ELISA Kit, CD13 ELISA Kit, GP150 ELISA Kit, LAP1 ELISA Kit, P150 ELISA Kit, PEPN ELISA Kit, A2AP ELISA Kit, AAP ELISA Kit, ALPHA-2-PI ELISA Kit, API ELISA Kit, PLI ELISA Kit, AAT1 ELISA Kit, ALT1 ELISA Kit, GPT1 ELISA Kit, Gpt1 ELISA Kit, Apn ELISA Kit, pepN ELISA Kit, SC8E4A.13 ELISA Kit, 1300007J06Rik ELISA Kit, 2310022B03Rik ELISA Kit, ALT ELISA Kit, Gpt-1 ELISA Kit, AP-M ELISA Kit, AP-N ELISA Kit, Cd13 ELISA Kit, Apm ELISA Kit, KZP ELISA Kit, Lap1 ELISA Kit, rAPN ELISA Kit, APDE ELISA Kit, p150 ELISA Kit, anpep ELISA Kit, fb64c05 ELISA Kit, wu:fb64c05 ELISA Kit, zgc:136771 ELISA Kit, alanyl aminopeptidase, membrane ELISA Kit, serpin family F member 2 ELISA Kit, glutamic--pyruvic transaminase ELISA Kit, aminopeptidase N ELISA Kit, aminopeptidase ELISA Kit, aminopeptidase n ELISA Kit, glutamic pyruvic transaminase, soluble ELISA Kit, alanyl (membrane) aminopeptidase ELISA Kit, membrane alanyl aminopeptidase ELISA Kit, alanyl (membrane) aminopeptidase b ELISA Kit, alanyl aminopeptidase, membrane L homeolog ELISA Kit, ANPEP ELISA Kit, SERPINF2 ELISA Kit, GPT ELISA Kit, Gpt ELISA Kit, Apn2 ELISA Kit, Apn1 ELISA Kit, SCO2643 ELISA Kit, pepN ELISA Kit, Shewana3_0897 ELISA Kit, Alvin_1024 ELISA Kit, Ndas_3413 ELISA Kit, Saut_1544 ELISA Kit, Fbal_1877 ELISA Kit, ML5_3424 ELISA Kit, Despr_1988 ELISA Kit, Mesop_5365 ELISA Kit, Anpep ELISA Kit, LOC101744250 ELISA Kit, anpepb ELISA Kit, anpep.L ELISA Kit
    UniProt
    P97449
    Pathways
    Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones
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