Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

FLT3 ELISA Kit

FLT3 Reaktivität: Maus Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Produktnummer ABIN6730592
  • Target Alle FLT3 ELISA Kits anzeigen
    FLT3 (Fms-Related tyrosine Kinase 3 (FLT3))
    Reaktivität
    • 9
    • 3
    • 1
    Maus
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Mouse Flt-3/Flk-2 ELISA Kit.
    Proben
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytische Methode
    Quantitative
    Spezifität
    This ELISA antibody pair recognizes Mouse Flt-3.
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Featured
    Zu unserem meistverkauften FLT3 ELISA Kit
    Top Product
    Discover our top product FLT3 ELISA Kit
  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Informationen zur Lagerung
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Haltbarkeit
    6 months
  • Target Alle FLT3 ELISA Kits anzeigen
    FLT3 (Fms-Related tyrosine Kinase 3 (FLT3))
    Andere Bezeichnung
    Flt-3 (Flk-2 (FLT3 Produkte)
    Synonyme
    FLT3 ELISA Kit, B230315G04 ELISA Kit, CD135 ELISA Kit, Flk-2 ELISA Kit, Flk2 ELISA Kit, Flt-3 ELISA Kit, Ly72 ELISA Kit, wmfl ELISA Kit, FLK-2 ELISA Kit, FLK2 ELISA Kit, STK1 ELISA Kit, fms related tyrosine kinase 3 ELISA Kit, FMS-like tyrosine kinase 3 ELISA Kit, fms-related tyrosine kinase 3 ELISA Kit, FLT3 ELISA Kit, Flt3 ELISA Kit
    UniProt
    Q00342
    Pathways
    RTK Signalweg
Sie sind hier:
Kundenservice