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CHI3L1 ELISA Kit
CHI3L1
Reaktivität: Maus
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Produktnummer ABIN6730660
Produktdetails CHI3L1 ELISA Kit
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Target
Alle CHI3L1 ELISA Kits anzeigen
CHI3L1
(Chitinase 3-Like 1 (Cartilage Glycoprotein-39) (CHI3L1))
Reaktivität
Alle Reaktivitäten für CHI3L1 ELISA Kits
Maus
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Applikation
ELISA
Verwendungszweck
Mouse Chitinase 3-like 1 ELISA Kit.
Proben
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Analytische Methode
Quantitative
Spezifität
This ELISA antibody pair recognizes Mouse Chitinase 3-like 1.
Produktmerkmale
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 µl to 1 µl volumes Adjustable 1-25 µl pipettes for reagent preparation 100 µl and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Anwendungsinformationen
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Applikationshinweise
Optimal working dilution should be determined by the investigator.
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 100 µl of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4°C. Add 100 µl of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 µl of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 µl of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 µl of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Informationen zur Lagerung
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit
6 months
Details zu CHI3L1
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Target
Alle CHI3L1 ELISA Kits anzeigen
CHI3L1
(Chitinase 3-Like 1 (Cartilage Glycoprotein-39) (CHI3L1))
Andere Bezeichnung
Chitinase 3-like 1 (CHI3L1 Produkte )
Synonyme
AW208766 ELISA Kit, Brp39 ELISA Kit, Gp39 ELISA Kit, ASRT7 ELISA Kit, CGP-39 ELISA Kit, GP-39 ELISA Kit, GP39 ELISA Kit, HC-gp39 ELISA Kit, HCGP-3P ELISA Kit, YKL-40 ELISA Kit, YKL40 ELISA Kit, YYL-40 ELISA Kit, hCGP-39 ELISA Kit, GP38K ELISA Kit, CLP-1 ELISA Kit, SPC-40 ELISA Kit, SPS-40 ELISA Kit, Chil1 ELISA Kit, BP40 ELISA Kit, MGP-40 ELISA Kit, chitinase-like 1 ELISA Kit, chitinase 3 like 1 ELISA Kit, chitinase 3-like 1 (cartilage glycoprotein-39) ELISA Kit, Chil1 ELISA Kit, CHI3L1 ELISA Kit, Chi3l1 ELISA Kit
Substanzklasse
Viral Protein
UniProt
Q61362
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