EPH Receptor A2 ELISA Kit

Produktnummer ABIN6730664

Produktdetails EPH Receptor A2 ELISA Kit

Target: EPH Receptor A2 (EPHA2)

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Mouse EphA2 ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair recognizes Mouse EphA2.

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu EPH Receptor A2

Target: EPH Receptor A2 (EPHA2)

Andere Bezeichnung: EphA2 (EPHA2 Produkte)

Synonyme: ARCC2 ELISA Kit, CTPA ELISA Kit, CTPP1 ELISA Kit, CTRCT6 ELISA Kit, ECK ELISA Kit, epha2 ELISA Kit, MGC89505 ELISA Kit, AW545284 ELISA Kit, Eck ELISA Kit, Myk2 ELISA Kit, Sek-2 ELISA Kit, Sek2 ELISA Kit, id:ibd5152 ELISA Kit, rtk6 ELISA Kit, G42 ELISA Kit, XE10 ELISA Kit, epha2-A ELISA Kit, EPH receptor A2 ELISA Kit, Eph receptor A2 ELISA Kit, epoxide hydrolase ELISA Kit, eph receptor A2 a ELISA Kit, EPH receptor A2 L homeolog ELISA Kit, EPHA2 ELISA Kit, Epha2 ELISA Kit, epha2 ELISA Kit, ephA2 ELISA Kit, epha2a ELISA Kit, epha2.L ELISA Kit

UniProt: Q03145

Pathways: RTK Signalweg