PFKM ELISA Kit

Produktnummer ABIN6730720

Produktdetails PFKM ELISA Kit

Target: PFKM (phosphofructokinase, Muscle (PFKM))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human Muscle Phosphofructokinase/PFKM ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair recognizes Human Muscle Phosphofructokinase.

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu PFKM

Target: PFKM (phosphofructokinase, Muscle (PFKM))

Andere Bezeichnung: Muscle Phosphofructokinase (PFKM (PFKM Produkte)

Synonyme: GSD7 ELISA Kit, PFK-1 ELISA Kit, PFK1 ELISA Kit, PFKA ELISA Kit, PFKX ELISA Kit, AI131669 ELISA Kit, PFK-A ELISA Kit, PFK-M ELISA Kit, Pfk-4 ELISA Kit, Pfk4 ELISA Kit, Pfka ELISA Kit, Pfkx ELISA Kit, Pfk-M ELISA Kit, M-PFK ELISA Kit, PFKM ELISA Kit, pfkm ELISA Kit, zgc:92344 ELISA Kit, pfk ELISA Kit, im:7137110 ELISA Kit, wu:fc58g11 ELISA Kit, phosphofructokinase, muscle ELISA Kit, phosphofructokinase, platelet ELISA Kit, phosphofructokinase, muscle a ELISA Kit, phosphofructokinase, muscle S homeolog ELISA Kit, phosphofructokinase, muscle b ELISA Kit, PFKM ELISA Kit, Pfkm ELISA Kit, PFKP ELISA Kit, pfkma ELISA Kit, pfkm.S ELISA Kit, pfkmb ELISA Kit

UniProt: P08237

Pathways: Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Warburg Effekt