EPH Receptor B2 ELISA Kit

Produktnummer ABIN6730732

Produktdetails EPH Receptor B2 ELISA Kit

Target: EPH Receptor B2 (EPHB2)

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Mouse EphB2 ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair recognizes Mouse EphB2.

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu EPH Receptor B2

Target: EPH Receptor B2 (EPHB2)

Andere Bezeichnung: EphB2 (EPHB2 Produkte)

Synonyme: CAPB ELISA Kit, DRT ELISA Kit, EK5 ELISA Kit, EPHT3 ELISA Kit, ERK ELISA Kit, Hek5 ELISA Kit, PCBC ELISA Kit, Tyro5 ELISA Kit, ERK2 ELISA Kit, ERT1 ELISA Kit, MAPK2 ELISA Kit, P42MAPK ELISA Kit, PRKM1 ELISA Kit, PRKM2 ELISA Kit, p38 ELISA Kit, p40 ELISA Kit, p41 ELISA Kit, p41mapk ELISA Kit, RGD1564232 ELISA Kit, Cek5 ELISA Kit, Drt ELISA Kit, ETECK ELISA Kit, Erk ELISA Kit, Nuk ELISA Kit, Prkm5 ELISA Kit, Qek5 ELISA Kit, Sek3 ELISA Kit, cek5 ELISA Kit, ephb2 ELISA Kit, xEphB2 ELISA Kit, si:ch73-160e9.1 ELISA Kit, tpx2 ELISA Kit, wu:fb09e10 ELISA Kit, wu:fc64a03 ELISA Kit, wu:fi18g12 ELISA Kit, zgc:112296 ELISA Kit, EPH receptor B2 ELISA Kit, mitogen-activated protein kinase 1 ELISA Kit, Eph receptor B2 ELISA Kit, EPH receptor B2 L homeolog ELISA Kit, eph receptor B2a ELISA Kit, eph receptor B2b ELISA Kit, EPHB2 ELISA Kit, MAPK1 ELISA Kit, Ephb2 ELISA Kit, ephb2.L ELISA Kit, ephb2a ELISA Kit, ephb2b ELISA Kit

UniProt: P54763

Pathways: RTK Signalweg, Regulation of long-term Neuronal Synaptic Plasticity, S100 Proteine