NPM1 ELISA Kit

Produktnummer ABIN6730755

Produktdetails NPM1 ELISA Kit

Target: NPM1 (Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human NPM1 ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair recognizes Human NPM1.

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu NPM1

Target: NPM1 (Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1))

Andere Bezeichnung: NPM1 (NPM1 Produkte)

Synonyme: B23 ELISA Kit, NPM ELISA Kit, NO38 ELISA Kit, Npm ELISA Kit, B23NP ELISA Kit, npm4 ELISA Kit, npm ELISA Kit, CG7917 ELISA Kit, CRP1 ELISA Kit, Crp1 ELISA Kit, Dmel\\CG7917 ELISA Kit, dNLP ELISA Kit, dNLP-S ELISA Kit, p22 ELISA Kit, npm1 ELISA Kit, sb:cb487 ELISA Kit, nucleophosmin 1 ELISA Kit, nucleophosmin (nucleolar phosphoprotein B23, numatrin) L homeolog ELISA Kit, nucleophosmin 1b ELISA Kit, Nucleophosmin ELISA Kit, nucleophosmin ELISA Kit, nucleophosmin (nucleolar phosphoprotein B23, numatrin) ELISA Kit, Nucleoplasmin ELISA Kit, nucleophosmin 1a ELISA Kit, NPM1 ELISA Kit, Npm1 ELISA Kit, npm1.L ELISA Kit, npm1b ELISA Kit, npm ELISA Kit, Nlp ELISA Kit, npm1a ELISA Kit

UniProt: P06748

Pathways: Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly